Immunohistochemical Expression of Estrogen and Progesterone Receptors in Human Colorectal Adenoma and Carcinoma Using Specified Automated Cellular Image Analysis System: A Clinicopathological Study

• Main Authors: Alaa Ghani Hussein, Hussam Hasson Ali, Ban Jumaa Qasim
• Format: Article
• Language: English
• Published: Oman Medical Specialty Board 2011-09-01
• Series: Oman Medical Journal
• Subjects: estrogen receptors; progesterone receptors; colorectal adenoma; Colorectal Carcinoma
• Online Access: http://journals.indexcopernicus.com/fulltxt.php?ICID=968866
• ISSN: 1999-768X; 2070-5204

 Objectives: To evaluate the immunohistochemical expression of estrogen receptors (ER) and progesterone receptors (PR) in colorectal adenoma and adenocarcinoma and to correlate this immunohistochemical expression with different clinicopathological parameters.Methods: The study was retrospectively designed. A total of 86 tissue samples, including 33 paraffin blocks from patients with colorectal adenomas, 33 paraffin blocks from patients with colorectal adenocarcinomas and a control group of 20 samples of non-tumorous colonic tissue, were included in the study.Results: The frequency of expression of ER and PR showed a gradual increase from control through adenoma to carcinoma. The frequencies of expression of ER in the control, adenoma and carcinoma were (10�2015.15�0and 42.42�0respectively, p<0.001), while the frequency of expression for PR were (10�2024.24�0and 36.36�0respectively, p<0.001). Strong ER and PR staining was mainly seen in carcinoma cases (42.42�2036.36�20respectively) in comparison with adenoma (9.09�2015.15�20respectively) and control (0�200�20respectively). The three digital parameters of ER and PR immunohistochemical expression (Area [A], Number of objects [N], and intensity [I]) were significantly increased in a sequence of normal mucosa-adenoma-carcinoma. There was a significant positive correlation between ER and PR in adenoma (r=0.312, p=0.034) and carcinoma (r=0.321, p=0.0398).Conclusion: ER and PR expression increased in a sequence of; normal colonic mucosa-adenoma-carcinoma, and a positive correlation was observed between ER and PR expression in colonic adenoma and carcinoma specimen indicating that ER and PR may play a role in colorectal carcinogenesis.