Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers

Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers

A recombinant 130 kDa dihemoglobin which is made up of a single-chain tetra-α globin and four β globins has been expressed as a soluble protein in E. coli. The sequence of the single chain tetra-α is: αI-Gly-αII-(SerGlyGly)5Ser-αIII-Gly-αIV. This dihemoglobin has been purified and characterized in v...

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Bibliographic Details
Main Authors: Jacqueline F. Aitken, Spencer J. Anthony-Cahill, Douglas D. Lemon, Michael P. Doyle, David A. Marquardt, Jeffrey S. Davidson, Janet K. Epp
Format: Article
Language:English
Published: MDPI AG 2012-01-01
Series:Journal of Functional Biomaterials
Subjects:
polyhemoglobin
dihemoglobin
tetrahemoglobin
hemoglobin engineering
extravasation
pressor response
Online Access:http://www.mdpi.com/2079-4983/3/1/61/

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