Expression and Clinical Significance of Cytoskeleton microtubule-associated - Tau in gastric carcinoma

• Main Authors: Chun-Hui Li, Meiling Hao, Rajina Sahi
• Format: Article
• Language: English
• Published: College of Medical Sciences 2015-12-01
• Series: Journal of College of Medical Sciences-Nepal
• Subjects: Gastric carcinoma, Cytoskeleton microtubule-associated-protein, Tau-protein, Reverse transcription polymerase chain reaction, immunohistochemistry
• Online Access: http://nepjol.info/index.php/JCMSN/article/view/14056

<p><strong>Background and Objectives: </strong>Microtubules, the main components of spindles in the mitotic phase, can provide the suitable conditions for unlimited proliferation of tumor cells. Cytoskeleton microtubule-associated-Tau accelerates the progress of malignant tumor through microtubules. Currently, the expression of Cytoskeleton microtubule-associated-Tau in gastric cancer and the relationship between clinical pathological factors research has not yet been reported, so the report aims to elucidate the features of Cytoskeleton microtubule-associated-Tau expression in normal gastric tissue and gastric carcinoma tissue.</p><p><strong>Materials and Methods: </strong>The expressions of Cytoskeleton microtubule-associated protein-Tau protein and Cytoskeleton microtubule-associated protein-Tau mRNA were investigated in 60 cases of gastric carcinoma (cases) and 10 cases of normal gastric tissues (controls). Immunohistochemistry and RT-PCR were respectively used to detect the expression of Cytoskeleton microtubule-associated protein-Tau protein and Tau mRNA in the normal and carcinomatous gastric tissues.</p><p><strong>Results: </strong>The expressions of both cytoskeleton microtubule-associated protein-Tau protein and Tau mRNA in the carcinomatous gastric tissue were higher than in the normal gastric tissue (<em>P&lt;0.05</em>). Likewise, both biomarkers were expressed significantly lower in the well-differentiated and moderately-differentiated gastric carcinoma was than in the poorly-differentiated one (<em>P&lt;0.05</em>). Moreover, their expressions in the gastric carcinoma without lymph node metastasis was lower than in the gastric carcinoma with lymph node metastasis (<em>P&lt;0.05</em>).</p><p><strong>Conclusion: </strong>The expressions of Cytoskeleton microtubule-associated-protein, Tau-protein and Cytoskeleton microtubule-associated-protein, Tau-mRNA were significantly different between the normal gastric tissue and the gastric carcinoma tissue, and were correlated with the degree differentiation and lymph node status.</p><p class="Default">JCMS Nepal. 2015;11(3):6-11.</p>