Effects of Some Insecticides on the Viability and the ATP Synthesis of Honeybee Drone’s Spermatozoid in vitro Exposed
Honeybee (Apis mellifera) reproduction is polyandrous: the queen obtains millions of spermatozoid by mating with several drones outside the colony. Fertility problems of honeybee queens are reported where failure of the production and quality of sperm drones are suspected. Several factors can affect...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institution of the Agricultural Research and Higher Education
2015-06-01
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| Series: | Tunisian Journal of Plant Protection |
| Subjects: | |
| Online Access: |
http://www.tjpp.tn/SiteWeb/PreviousIssues/TJPP10-1/10BenAbdelkader.pdf
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| Summary: | Honeybee (Apis mellifera) reproduction is polyandrous: the queen obtains millions of spermatozoid by
mating with several drones outside the colony. Fertility problems of honeybee queens are reported
where failure of the production and quality of sperm drones are suspected. Several factors can affect
sperm quality drones include pesticides. The aim of this study is to determinate the in vitro effect of
fipronil, ethiprole, imidacloprid, thiamethoxam, cypermethrin, and coumaphos at different
concentrations from 0.1 to 100 µM on the viability and the energetic state, through ATP content, of
spermatozoids of honeybee drones. Exposure during 24 h showed that all the active ingredients used in
this test increase the ATP levels. Four of them i.e. fipronil, ethiprole, imidacloprid, and thiamethoxam
reduced significantly the viability of spermatozoids. Hence, pesticides could affect the drone’s
spermatozoids which may have negative impact on semen quality and further queen fecundity. |
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| ISSN: | 1737-5436 2490-4368 |