Isolation of High Molecular Weight DNA from the Model Beetle <i>Tribolium</i> for Nanopore Sequencing

Isolation of High Molecular Weight DNA from the Model Beetle <i>Tribolium</i> for Nanopore Sequencing

The long-read Nanopore sequencing has been recently applied for assembly of complex genomes and analysis of linear genome organization. The most critical factor for successful long-read sequencing is extraction of high molecular weight (HMW) DNA of sufficient purity and quantity. The challenges asso...

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Bibliographic Details
Main Authors: Marin Volarić, Damira Veseljak, Brankica Mravinac, Nevenka Meštrović, Evelin Despot-Slade
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Genes
Subjects:
beetle
high molecular weight DNA
Nanopore sequencing
Online Access:https://www.mdpi.com/2073-4425/12/8/1114

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