Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from Amazon lichens

• Main Authors: Germana M.M. Silva, Raquel P. Bezerra, José A. Teixeira, Tatiana S. Porto, José L. Lima-Filho, Ana Lúcia F. Porto
• Format: Article
• Language: English
• Published: Elsevier 2015-01-01
• Series: Electronic Journal of Biotechnology
• Subjects: Actinomycetes; Factorial design; Glucose; Microbial enzymes; Soybean flour
• Online Access: http://www.sciencedirect.com/science/article/pii/S0717345814001389

Background: Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results: The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm2 was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions: These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.