Histological changes of cerebrum and spinal cord after thermal injury and lyophilized xenographs usage

• Main Authors: Volkov K.S., Dowbush А.V., Karpenyuk V.M.
• Format: Article
• Language: Russian
• Published: Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy 2008-01-01
• Series: Морфологія
• Subjects: cerebrum; spinal cord; histological changes; ultrastructure; thermal trauma; burn; lyophilized xenographs
• Online Access: http://www.morphology.dp.ua/_pub/MORPHO-2008-02-01/08vkszlk.pdf

The objective of research was establishment of the histological state of cereblal cortex and spinal cord by means of using thermal trauma and xenographs. Experiments have been made on 24 sexually active guinea pigs with a burn trauma and animals which burn injuries were covered by lyophilized xenographs. Histological specimens were stained with toluidin blue by Nissl’s method, electronmicroscopic slices were fixed with glutaraldehid, post-fixed with tetraokis osmium and contrasted plumbum citrate by Reynolds’. In experiment on guinea pigs there have been investigated histological state of cerebral cortex and spinal cord after thermal injury of skin in the circumstances of carrying out early necrectomy and covering the wounds by lyophilized xenographs. Microscopic and elecronmicroscopic investigations were made and showed deep changes of neurons and vessels bed during thermal trauma. There also has been revealed positive influence of xenographs usage in the course of regenerative progresses and reestablishment of organ structural compounds of central nerve system in dynamics of experiment.