Norovirus Outbreaks from Drinking Water

As part of an intensified monitoring program for foodborne disease outbreaks in Finland, waterborne outbreaks were investigated for viruses. The diagnostic procedure included analysis of patients' stool samples by electron microscopy and reverse transcription–polymerase chain reaction (RT-PCR)...

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Main Authors: Leena Maunula, Ilkka T. Miettinen, Carl-Henrik von Bonsdorff
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2005-11-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/11/11/05-0487_article
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spelling doaj-b65f0ed614c04a6882961a9eb2e141ab2020-11-24T22:15:44ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592005-11-0111111716172110.3201/eid1111.050487Norovirus Outbreaks from Drinking WaterLeena MaunulaIlkka T. MiettinenCarl-Henrik von BonsdorffAs part of an intensified monitoring program for foodborne disease outbreaks in Finland, waterborne outbreaks were investigated for viruses. The diagnostic procedure included analysis of patients' stool samples by electron microscopy and reverse transcription–polymerase chain reaction (RT-PCR) for noroviruses and astroviruses. When these test results were positive for a virus, the water sample was analyzed. Virus concentration was based on positively charged filters from 1-L samples. Of the total 41 waterborne outbreaks reported during the observation period (1998–2003), samples from 28 outbreaks were available for analysis. As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks. In 10 outbreaks, the water sample also yielded a norovirus. In all but 1 instance, the amplicon sequence was identical to that recovered from the patients. The ubiquity of waterborne norovirus outbreaks calls for measures to monitor water for viruses.https://wwwnc.cdc.gov/eid/article/11/11/05-0487_articleNorovirusgastroenteritisreverse transcriptase polymerase chain reactiongenotypedisease outbreaksresearch
collection DOAJ
language English
format Article
sources DOAJ
author Leena Maunula
Ilkka T. Miettinen
Carl-Henrik von Bonsdorff
spellingShingle Leena Maunula
Ilkka T. Miettinen
Carl-Henrik von Bonsdorff
Norovirus Outbreaks from Drinking Water
Emerging Infectious Diseases
Norovirus
gastroenteritis
reverse transcriptase polymerase chain reaction
genotype
disease outbreaks
research
author_facet Leena Maunula
Ilkka T. Miettinen
Carl-Henrik von Bonsdorff
author_sort Leena Maunula
title Norovirus Outbreaks from Drinking Water
title_short Norovirus Outbreaks from Drinking Water
title_full Norovirus Outbreaks from Drinking Water
title_fullStr Norovirus Outbreaks from Drinking Water
title_full_unstemmed Norovirus Outbreaks from Drinking Water
title_sort norovirus outbreaks from drinking water
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2005-11-01
description As part of an intensified monitoring program for foodborne disease outbreaks in Finland, waterborne outbreaks were investigated for viruses. The diagnostic procedure included analysis of patients' stool samples by electron microscopy and reverse transcription–polymerase chain reaction (RT-PCR) for noroviruses and astroviruses. When these test results were positive for a virus, the water sample was analyzed. Virus concentration was based on positively charged filters from 1-L samples. Of the total 41 waterborne outbreaks reported during the observation period (1998–2003), samples from 28 outbreaks were available for analysis. As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks. In 10 outbreaks, the water sample also yielded a norovirus. In all but 1 instance, the amplicon sequence was identical to that recovered from the patients. The ubiquity of waterborne norovirus outbreaks calls for measures to monitor water for viruses.
topic Norovirus
gastroenteritis
reverse transcriptase polymerase chain reaction
genotype
disease outbreaks
research
url https://wwwnc.cdc.gov/eid/article/11/11/05-0487_article
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