Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan

Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan

Green toadfish Lagocephalus lunaris inhabits tropical and subtropical seas and contains high tetrodotoxin (TTX) levels in the muscle as well as liver and gonad. In 2008 to 2009, food poisoning due to ingesting L. lunais occurred in Western Japan. Five specimens of green toadfish caught in Kyushu coa...

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Main Authors: Yuji Nagashima, Takuya Matsumoto, Keisuke Kadoyama, Shoichiro Ishizaki, Makoto Terayama
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Journal of Toxicology
Online Access:http://dx.doi.org/10.1155/2011/801285
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spelling doaj-b630b8691b144fe1b2d8ab5287226c5a2020-11-24T23:03:43ZengHindawi LimitedJournal of Toxicology1687-81911687-82052011-01-01201110.1155/2011/801285801285Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, JapanYuji Nagashima0Takuya Matsumoto1Keisuke Kadoyama2Shoichiro Ishizaki3Makoto Terayama4Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, JapanAquaculture and Food Technology Division, Miyazaki Prefectural Fisheries Experimental Station, Aoshima 6-13-6, Miyazaki 889-2162, JapanGreen toadfish Lagocephalus lunaris inhabits tropical and subtropical seas and contains high tetrodotoxin (TTX) levels in the muscle as well as liver and gonad. In 2008 to 2009, food poisoning due to ingesting L. lunais occurred in Western Japan. Five specimens of green toadfish caught in Kyushu coast, Japan, were analyzed for toxicity, toxins, and species identification. All five specimens were toxic by bioassay. Comparing the maximum toxicity in tissues, ovary contained the most toxin (1810 mouse unit [MU]/g), followed by liver (341 MU/g), muscle (135 MU/g), skin (79 MU/g), and intestine (72 MU/g). Liquid chromatography/mass spectrometry analysis revealed that TTX was the major toxin. Nucleotide sequence analysis of the 16S rRNA gene fragment of muscle mitochondrial DNA indicated that partial sequences of PCR products of four specimens were identical with that of L. lunaris. The sequence of one specimen was indistinguishable from that of the brown-backed toadfish Lagocephalus wheeleri, a nontoxic species.http://dx.doi.org/10.1155/2011/801285
collection DOAJ
language English
format Article
sources DOAJ
author Yuji Nagashima
Takuya Matsumoto
Keisuke Kadoyama
Shoichiro Ishizaki
Makoto Terayama
spellingShingle Yuji Nagashima
Takuya Matsumoto
Keisuke Kadoyama
Shoichiro Ishizaki
Makoto Terayama
Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
Journal of Toxicology
author_facet Yuji Nagashima
Takuya Matsumoto
Keisuke Kadoyama
Shoichiro Ishizaki
Makoto Terayama
author_sort Yuji Nagashima
title Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
title_short Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
title_full Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
title_fullStr Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
title_full_unstemmed Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan
title_sort toxicity and molecular identification of green toadfish lagocephalus lunaris collected from kyushu coast, japan
publisher Hindawi Limited
series Journal of Toxicology
issn 1687-8191
1687-8205
publishDate 2011-01-01
description Green toadfish Lagocephalus lunaris inhabits tropical and subtropical seas and contains high tetrodotoxin (TTX) levels in the muscle as well as liver and gonad. In 2008 to 2009, food poisoning due to ingesting L. lunais occurred in Western Japan. Five specimens of green toadfish caught in Kyushu coast, Japan, were analyzed for toxicity, toxins, and species identification. All five specimens were toxic by bioassay. Comparing the maximum toxicity in tissues, ovary contained the most toxin (1810 mouse unit [MU]/g), followed by liver (341 MU/g), muscle (135 MU/g), skin (79 MU/g), and intestine (72 MU/g). Liquid chromatography/mass spectrometry analysis revealed that TTX was the major toxin. Nucleotide sequence analysis of the 16S rRNA gene fragment of muscle mitochondrial DNA indicated that partial sequences of PCR products of four specimens were identical with that of L. lunaris. The sequence of one specimen was indistinguishable from that of the brown-backed toadfish Lagocephalus wheeleri, a nontoxic species.
url http://dx.doi.org/10.1155/2011/801285
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