Design, synthesis and application of fluorogenic probe for detecting l-asparaginase in serum samples

• Main Authors: Lan Wang, Wenjing Qin, Ding Chen, Nanxiang Wang, Congcong Zhang, Zhijie Fang, Bin Fang, Wei Du, Naidi Yang, Qiong Wu, Bo Peng, Lin Li
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Results in Chemistry
• Subjects: Fluorogenic probe; l-asparaginase; In vitro assay; Patient serum
• Online Access: http://www.sciencedirect.com/science/article/pii/S2211715621000084
• ISSN: 2211-7156

Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro l-asparaginase detection. TPAN-Asn exhibited selective and robust response to l-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of l-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of l-asparaginase-based cancer therapy.