REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus

REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus

Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identificat...

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Bibliographic Details
Main Authors: K. V. Starostin, E. A. Demidov, A. S. Rozanov, A. V. Bryanskaya, S. E. Peltek
Format: Article
Language:English
Published: Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2015-01-01
Series:Vavilovskij Žurnal Genetiki i Selekcii
Subjects:
maldi
direct protein profiling
microorganism identification
geobacillus stearothermоphilus
Online Access:https://vavilov.elpub.ru/jour/article/view/198
Description
Summary:Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.
ISSN:2500-0462
2500-3259

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