Study of microflora composition of highly efficient bacterial strains capable of degrading pig hair keratin and the effects of degradation
This study aimed to enrich the microbial resource pool of porcine keratin-degrading bacterial strains and provide technical support for the generation of effective degradation products from porcine keratin. In this study, an enriched, domesticated culture method was used, where pig hair keratin-degr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Agro-Environmental Protection Institute, Ministry of Agriculture
2020-11-01
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| Series: | Journal of Agricultural Resources and Environment |
| Subjects: | |
| Online Access: | http://www.aed.org.cn/nyzyyhjxb/html/2020/6/20200623.htm |
| Summary: | This study aimed to enrich the microbial resource pool of porcine keratin-degrading bacterial strains and provide technical support for the generation of effective degradation products from porcine keratin. In this study, an enriched, domesticated culture method was used, where pig hair keratin-degrading strains were screened from soil using pig hair as the sole carbon and nitrogen source. A combination of different strains was used to prepare combined flora. Three strains of pig hair keratin-degrading bacteria with broadspectrum keratin-degrading capabilities were screened. The pig hair degradation rate(10 d)of three strains were 58.0%~65.3% with good degradation of chicken feathers, goose feathers, and wool. Three highly efficient strains that could degrade pig hair(strain numbers, E-2-2, E-1-4, and E-3-2) were identified to be <i>Bacillus licheniformis, Bacillus mycoides</i>, and <i>Streptomyces koyangensis</i>, respectively. By comparing and analyzing degradation effects of each strain and the different combinations of flora, an optimal flora combination was obtained. The degradation rate of pig hair(10 d)with the combined flora was as high as 81.8%, i.e., 23.8 percent points higher than the degradation rate with a single strain(10 d). During the degradation process, a large amount of soluble protein was produced. Furthermore, sulfur in the disulfide bonds of keratin was converted to sulfur-containing compounds with sulfate being the main transformation product. The synergistic effect of the different strains may greatly improve degradation of pig hair, and thus, has potential to be applied in the production of amino acid-based feed. |
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| ISSN: | 2095-6819 2095-6819 |