Anomalous blood grouping showing red cell mosaicism in a patient with acute leukemia

• Main Authors: Sanmukh Ratilal Joshi, Komal Savaliya, Manisha Rajapara, Parul Narang
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2018-01-01
• Series: Global Journal of Transfusion Medicine
• Subjects: Anomalous blood grouping; leukemia; red blood cell mosaicism
• Online Access: http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2018;volume=3;issue=1;spage=59;epage=61;aulast=Joshi

Background: The ABO blood groups are determined by forward grouping on red cells and are confirmed by reverse grouping on serum, both of which must tally to assign proper blood group to an individual. Any anomaly in blood group must resolve before transfusion. Aim: A patient with anomalous blood grouping required to be investigated as pretransfusion tests. Materials and Methods: ABO blood grouping protocol was followed. The patient's red blood cells (RBCs), showing mixed-field reaction, were separated into those showing agglutination from other remaining nonagglutinated RBCs by selective adsorption using anti-A1 lectin and subsequently disagglutinated by incubating with soluble salivary antigen from Group A secretor individual. Results: Of the two RBCs populations, one that was agglutinated by the lectin showed considerably stronger agglutination with anti-A antibody as compared to those that were not agglutinated. Conclusion: Weakened red cell A antigen was presumed to be due to underlying disease process in acute leukemia.