Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1

Laryngeal squamous cell carcinoma (LSCC) arises from the squamous epithelium of the larynx and is associated with a high incidence of cervical lymph node metastasis. MicroRNAs (miRNAs) play a crucial role in the epigenetic regulation of cellular biological processes, including cancer metastasis. How...

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Main Authors: Xin Li, Ping Wu, Yaoyun Tang, Yuhua Fan, Yalan Liu, Xing Fang, Wei Wang, Suping Zhao
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Oncology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fonc.2020.544476/full
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language English
format Article
sources DOAJ
author Xin Li
Xin Li
Xin Li
Ping Wu
Ping Wu
Ping Wu
Yaoyun Tang
Yaoyun Tang
Yaoyun Tang
Yuhua Fan
Yuhua Fan
Yuhua Fan
Yalan Liu
Yalan Liu
Yalan Liu
Xing Fang
Xing Fang
Xing Fang
Wei Wang
Wei Wang
Wei Wang
Suping Zhao
Suping Zhao
Suping Zhao
spellingShingle Xin Li
Xin Li
Xin Li
Ping Wu
Ping Wu
Ping Wu
Yaoyun Tang
Yaoyun Tang
Yaoyun Tang
Yuhua Fan
Yuhua Fan
Yuhua Fan
Yalan Liu
Yalan Liu
Yalan Liu
Xing Fang
Xing Fang
Xing Fang
Wei Wang
Wei Wang
Wei Wang
Suping Zhao
Suping Zhao
Suping Zhao
Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
Frontiers in Oncology
miRNA
mRNA
laryngeal squamous cell carcinoma
epithelial-to-mesenchymal transition
biomarker
author_facet Xin Li
Xin Li
Xin Li
Ping Wu
Ping Wu
Ping Wu
Yaoyun Tang
Yaoyun Tang
Yaoyun Tang
Yuhua Fan
Yuhua Fan
Yuhua Fan
Yalan Liu
Yalan Liu
Yalan Liu
Xing Fang
Xing Fang
Xing Fang
Wei Wang
Wei Wang
Wei Wang
Suping Zhao
Suping Zhao
Suping Zhao
author_sort Xin Li
title Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
title_short Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
title_full Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
title_fullStr Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
title_full_unstemmed Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1
title_sort down-regulation of mir-181c-5p promotes epithelial-to-mesenchymal transition in laryngeal squamous cell carcinoma via targeting serpine1
publisher Frontiers Media S.A.
series Frontiers in Oncology
issn 2234-943X
publishDate 2020-12-01
description Laryngeal squamous cell carcinoma (LSCC) arises from the squamous epithelium of the larynx and is associated with a high incidence of cervical lymph node metastasis. MicroRNAs (miRNAs) play a crucial role in the epigenetic regulation of cellular biological processes, including cancer metastasis. However, the molecular mechanisms of specific miRNAs responsible for LSCC metastasis and their clinical significance have yet to be fully elucidated. In this study, LSCC cohort datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) were downloaded and examined by comprehensive bioinformatics analysis, which revealed that upregulation of mRNA SERPINE1 and downregulation of miR-181c-5p were associated with unfavorable overall survival. Our analysis showed that SERPINE1 expression negatively correlated with the expression level of miR-181c-5p in our LSCC patient samples. Silencing of miR-181c-5p expression promoted cell migration and invasion in cell lines, whereas the overexpression of miR-181c-5p suppressed cell migration and epithelial-to-mesenchymal transition (EMT) through the downregulation of SERPINE1. Further analysis showed that the enhancement effect on EMT and metastasis induced by silencing miR-181c-5p could be rescued through knockdown of SERPINE1 expression in vitro. Collectively, our findings indicated that miR-181c-5p acted as an EMT suppressor miRNA by downregulation of SERPINE1 in LSCC and offers novel strategies for the prevention of metastasis in LSCC.
topic miRNA
mRNA
laryngeal squamous cell carcinoma
epithelial-to-mesenchymal transition
biomarker
url https://www.frontiersin.org/articles/10.3389/fonc.2020.544476/full
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spelling doaj-b57f4cf3a34a455fb977d7104f811b012021-01-13T16:04:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-12-011010.3389/fonc.2020.544476544476Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1Xin Li0Xin Li1Xin Li2Ping Wu3Ping Wu4Ping Wu5Yaoyun Tang6Yaoyun Tang7Yaoyun Tang8Yuhua Fan9Yuhua Fan10Yuhua Fan11Yalan Liu12Yalan Liu13Yalan Liu14Xing Fang15Xing Fang16Xing Fang17Wei Wang18Wei Wang19Wei Wang20Suping Zhao21Suping Zhao22Suping Zhao23Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, ChinaProvince Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, ChinaLaryngeal squamous cell carcinoma (LSCC) arises from the squamous epithelium of the larynx and is associated with a high incidence of cervical lymph node metastasis. MicroRNAs (miRNAs) play a crucial role in the epigenetic regulation of cellular biological processes, including cancer metastasis. However, the molecular mechanisms of specific miRNAs responsible for LSCC metastasis and their clinical significance have yet to be fully elucidated. In this study, LSCC cohort datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) were downloaded and examined by comprehensive bioinformatics analysis, which revealed that upregulation of mRNA SERPINE1 and downregulation of miR-181c-5p were associated with unfavorable overall survival. Our analysis showed that SERPINE1 expression negatively correlated with the expression level of miR-181c-5p in our LSCC patient samples. Silencing of miR-181c-5p expression promoted cell migration and invasion in cell lines, whereas the overexpression of miR-181c-5p suppressed cell migration and epithelial-to-mesenchymal transition (EMT) through the downregulation of SERPINE1. Further analysis showed that the enhancement effect on EMT and metastasis induced by silencing miR-181c-5p could be rescued through knockdown of SERPINE1 expression in vitro. Collectively, our findings indicated that miR-181c-5p acted as an EMT suppressor miRNA by downregulation of SERPINE1 in LSCC and offers novel strategies for the prevention of metastasis in LSCC.https://www.frontiersin.org/articles/10.3389/fonc.2020.544476/fullmiRNAmRNAlaryngeal squamous cell carcinomaepithelial-to-mesenchymal transitionbiomarker