Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand

Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand

<abstract language="eng">NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery. Among the recent NMR techniques to probe weak binding protein-ligand complexes...

Full description

Bibliographic Details
Main Authors: Isis Martins Figueiredo, Anita Jocelyne Marsaioli
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2007-01-01
Series:Química Nova
Subjects:
NMR
protein-ligand interactions
bovine serum albumin
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000700019
id doaj-b57d1907c2fa455eacafcc10107fa717
record_format Article
spelling doaj-b57d1907c2fa455eacafcc10107fa7172020-11-24T22:12:56ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642007-01-013071597160510.1590/S0100-40422007000700019Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligandIsis Martins FigueiredoAnita Jocelyne Marsaioli<abstract language="eng">NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery. Among the recent NMR techniques to probe weak binding protein-ligand complexes we have critically evaluated the advantages and disadvantages of STD (Saturation Transfer Difference), WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy), NOE pumping and DOSY-NOESY (Diffusion-Ordered NOESY) using a mixture of BSA (bovine serum albumin) plus salicylic acid, caffeine, citric acid, adipic acid and D-glucose.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000700019NMRprotein-ligand interactionsbovine serum albumin
collection DOAJ
language English
format Article
sources DOAJ
author Isis Martins Figueiredo
Anita Jocelyne Marsaioli
spellingShingle Isis Martins Figueiredo
Anita Jocelyne Marsaioli
Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
Química Nova
NMR
protein-ligand interactions
bovine serum albumin
author_facet Isis Martins Figueiredo
Anita Jocelyne Marsaioli
author_sort Isis Martins Figueiredo
title Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
title_short Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
title_full Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
title_fullStr Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
title_full_unstemmed Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand
title_sort mapeamento das interações proteína-ligante através de técnicas de rmn de ¹h utilizando detecção do ligante screening protein-ligand interactions using ¹h nmr techniques for detecting the ligand
publisher Sociedade Brasileira de Química
series Química Nova
issn 0100-4042
1678-7064
publishDate 2007-01-01
description <abstract language="eng">NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery. Among the recent NMR techniques to probe weak binding protein-ligand complexes we have critically evaluated the advantages and disadvantages of STD (Saturation Transfer Difference), WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy), NOE pumping and DOSY-NOESY (Diffusion-Ordered NOESY) using a mixture of BSA (bovine serum albumin) plus salicylic acid, caffeine, citric acid, adipic acid and D-glucose.
topic NMR
protein-ligand interactions
bovine serum albumin
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000700019
work_keys_str_mv AT isismartinsfigueiredo mapeamentodasinteracoesproteinaliganteatravesdetecnicasdermnde1hutilizandodeteccaodoligantescreeningproteinligandinteractionsusing1hnmrtechniquesfordetectingtheligand
AT anitajocelynemarsaioli mapeamentodasinteracoesproteinaliganteatravesdetecnicasdermnde1hutilizandodeteccaodoligantescreeningproteinligandinteractionsusing1hnmrtechniquesfordetectingtheligand
_version_ 1725801931643813888

Similar Items