Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay

Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay

Summary: We describe a fluorescence recovery after photobleaching (FRAP) protocol for assessing the dynamics of heterochromatin/euchromatin and identifying chromatin relaxers for cell fate transition. Here, we developed a system to track heterochromatin foci with HP1α-cherry and performed FRAP assay...

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Bibliographic Details
Main Authors:	Qi Long, Juntao Qi, Wei Li, Yanshuang Zhou, Keshi Chen, Hao Wu, Xingguo Liu
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell-based Assays Microscopy Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721004135

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