Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

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In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single...

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Bibliographic Details
Main Authors: TOPIK HIDAYAT, ADI PANCORO
Format: Article
Language:English
Published: Bogor Agricultural University 2010-03-01
Series:Hayati Journal of Biosciences
Subjects:
homogenous sequences
internal transcribed spacer region
molecular phylogenetic
SSCP method
Online Access:http://www.sciencedirect.com/science/article/pii/S197830191630184X
Description
Summary:In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.
ISSN:1978-3019

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