A novel fluorescent pH probe for expression in plants

A novel fluorescent pH probe for expression in plants

<p>Abstract</p> <p>Background</p> <p>The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most po...

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Main Authors: Böttcher Markus, Lorenzen Inken, Schulte Alexander, Plieth Christoph
Format: Article
Language:English
Published: BMC 2006-04-01
Series:Plant Methods
Online Access:http://www.plantmethods.com/content/2/1/7
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spelling doaj-b54553f1eb344ce5b8c5eed2f15aeb382020-11-24T22:38:50ZengBMCPlant Methods1746-48112006-04-0121710.1186/1746-4811-2-7A novel fluorescent pH probe for expression in plantsBöttcher MarkusLorenzen InkenSchulte AlexanderPlieth Christoph<p>Abstract</p> <p>Background</p> <p>The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular <it>Aequorea victoria </it>GFP (<it>Av-</it>GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen <it>Ptilosarcus gurneyi </it>(<it>Pt-</it>GFP) and compare its properties with pHluorins for expression and use in plants.</p> <p>Results</p> <p>pHluorins have a higher pH-sensitivity. However, <it>Pt-</it>GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how <it>Pt</it>-GFP expressing <it>Arabidopsis thaliana </it>report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.</p> <p>Conclusion</p> <p><it>Pt-</it>GFP appears to be the better choice when used for <it>in vivo-</it>recording of cellular pH in plants.</p> http://www.plantmethods.com/content/2/1/7
collection DOAJ
language English
format Article
sources DOAJ
author Böttcher Markus
Lorenzen Inken
Schulte Alexander
Plieth Christoph
spellingShingle Böttcher Markus
Lorenzen Inken
Schulte Alexander
Plieth Christoph
A novel fluorescent pH probe for expression in plants
Plant Methods
author_facet Böttcher Markus
Lorenzen Inken
Schulte Alexander
Plieth Christoph
author_sort Böttcher Markus
title A novel fluorescent pH probe for expression in plants
title_short A novel fluorescent pH probe for expression in plants
title_full A novel fluorescent pH probe for expression in plants
title_fullStr A novel fluorescent pH probe for expression in plants
title_full_unstemmed A novel fluorescent pH probe for expression in plants
title_sort novel fluorescent ph probe for expression in plants
publisher BMC
series Plant Methods
issn 1746-4811
publishDate 2006-04-01
description <p>Abstract</p> <p>Background</p> <p>The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular <it>Aequorea victoria </it>GFP (<it>Av-</it>GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen <it>Ptilosarcus gurneyi </it>(<it>Pt-</it>GFP) and compare its properties with pHluorins for expression and use in plants.</p> <p>Results</p> <p>pHluorins have a higher pH-sensitivity. However, <it>Pt-</it>GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how <it>Pt</it>-GFP expressing <it>Arabidopsis thaliana </it>report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.</p> <p>Conclusion</p> <p><it>Pt-</it>GFP appears to be the better choice when used for <it>in vivo-</it>recording of cellular pH in plants.</p>
url http://www.plantmethods.com/content/2/1/7
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AT lorenzeninken anovelfluorescentphprobeforexpressioninplants
AT schultealexander anovelfluorescentphprobeforexpressioninplants
AT pliethchristoph anovelfluorescentphprobeforexpressioninplants
AT bottchermarkus novelfluorescentphprobeforexpressioninplants
AT lorenzeninken novelfluorescentphprobeforexpressioninplants
AT schultealexander novelfluorescentphprobeforexpressioninplants
AT pliethchristoph novelfluorescentphprobeforexpressioninplants
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