Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.

Bibliographic Details
Main Authors: C. D. Richardson, G. J. Ray, N. L. Bray, J. E. Corn
Format: Article
Language:English
Published: Nature Publishing Group 2016-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms12463
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spelling doaj-b52b9879e5a54a13a3e11a78f480fe3a2021-05-11T11:23:19ZengNature Publishing GroupNature Communications2041-17232016-08-01711710.1038/ncomms12463Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomesC. D. Richardson0G. J. Ray1N. L. Bray2J. E. Corn3Innovative Genomics Initiative, University of CaliforniaInnovative Genomics Initiative, University of CaliforniaInnovative Genomics Initiative, University of CaliforniaInnovative Genomics Initiative, University of CaliforniaCRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.https://doi.org/10.1038/ncomms12463
collection DOAJ
language English
format Article
sources DOAJ
author C. D. Richardson
G. J. Ray
N. L. Bray
J. E. Corn
spellingShingle C. D. Richardson
G. J. Ray
N. L. Bray
J. E. Corn
Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
Nature Communications
author_facet C. D. Richardson
G. J. Ray
N. L. Bray
J. E. Corn
author_sort C. D. Richardson
title Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_short Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_full Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_fullStr Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_full_unstemmed Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_sort non-homologous dna increases gene disruption efficiency by altering dna repair outcomes
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2016-08-01
description CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.
url https://doi.org/10.1038/ncomms12463
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AT gjray nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes
AT nlbray nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes
AT jecorn nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes
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