Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyti...

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Bibliographic Details
Main Authors: Arash Ghodousi, S Vatani, Davood Darban-Sarokhalil, Maryam Omrani, AA Fooladi, Ad Khosaravi, Mohammad Mehdi Feizabadi
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2012-03-01
Series:Iranian Journal of Microbiology
Subjects:
Mycobacterium tuberculosis
Pulsed-filed gel electrophoresis
TB-complex
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/713
Description
Summary:A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
ISSN:2008-3289
2008-4447

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