Si permeability of a deficient Lsi1 aquaporin in tobacco can be enhanced through a conserved residue substitution

• Main Authors: Devrim Coskun, Rupesh Deshmukh, Humira Sonah, Sheelavanta Matha Shivaraj, Rachelle Frenette‐Cotton, Laurence Tremblay, Paul Isenring, Richard R. Bélanger
• Format: Article
• Language: English
• Published: Wiley 2019-08-01
• Series: Plant Direct
• Subjects: aquaporin; influx; Lsi1; molecular determinants; NIP2‐1; silicon (Si)
• Online Access: https://doi.org/10.1002/pld3.163

Abstract Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si‐permeable Lsi1 (NIP2‐1) aquaporin in its roots. Structure‐function analyses of Lsi1 channels from higher plants have thus far revealed two key molecular determinants of Si permeability: (a) the amino acid motif GSGR in the aromatic/arginine selectivity filter and (b) 108 amino acids between two highly conserved NPA domains. Curiously, tobacco (Nicotiana sylvestris) stands as a rare exception as it possesses an Lsi1 (NsLsi1) with these molecular signatures but is reported as a low Si accumulator. The aim of this study was therefore to identify whether additional determinants influence Si permeability via Lsi1 channels, focusing on the role of residues that differ uniquely in NsLsi1 relative to functional Lsi1 homologs. We observed tobacco indeed absorbed Si poorly (0.1% dw), despite NsLsi1 being expressed constitutively in planta. Si influx measured in NsLsi1‐expressing Xenopus oocytes was very low (<13% that of OsLsi1 from rice (Oryza sativa) over a 3‐hr time course), which likely explains why tobacco is a low Si accumulator. Interestingly, NsLsi1P125F displayed a significant gain of function (threefold increase in Si influx relative to NsLsi1WT), which coincided with a threefold increase in plasma membrane localization in planta, as measured by transient expression of GFP constructs in Nicotiana benthamiana leaves. These findings thus reveal a novel molecular determinant of Si transport in plants and inform breeding, biotechnological, and agricultural practices to effectively utilize Si in the context of plant resilience to environmental stress.