Annealing dependent synthesis of cyto-compatible nano-silver/calcium hydroxyapatite composite for antimicrobial activities

• Main Authors: Arumugam Dhanesh Gandhi, K. Kaviyarasu, Nookala Supraja, Rajendran Velmurugan, Gunasekaran Suriyakala, Ranganathan Babujanarthanam, Yang Zang, Khantong Soontarapa, Khalid S. Almaary, Mohamed S. Elshikh, Tse-Wei Chen
• Format: Article
• Language: English
• Published: Elsevier 2021-11-01
• Series: Arabian Journal of Chemistry
• Subjects: Hydroxyapatite; Ureolytic Bacillus sp.; Synthetic urine; Antibacterial activity; Anticancer studies; Brine shrimp assay
• Online Access: http://www.sciencedirect.com/science/article/pii/S1878535221004196

Crystalline hydroxyapatite were synthesized from synthetic/human urine through precipitation which were further doped with silver nanoparticle for effective biomedical application. The aim were to improve overall biological compatibility of the synthesized bone-graft material even in oncogenesis cases. The thermal calcinated material was characterized by several techniques including UV–vis, Laser Raman, Fourier transmittance infrared spectroscopy, X-ray diffraction analysis, Transmission Eelectron microscopy and X-ray fluorescence spectroscopy. The quantitative and qualitative analysis revealed that the synthesized material was highly crystalline and nanosized with majority of silver and phosphate components. The antibacterial, anticancer and invitro cytotoxicity of the synthesized material was evaluated with Escherichia coli, Hela cells and brine shrimp assay, respectively. The brine shrimp assay revealed that the synthesized material is compatible with biological system, whereas anticancer activity showed the application of the synthesized biomaterial in cancer treatment in which antibacterial activity adds more advantage on preventing the bone-graft from microbial attack.