Greenhouse evaluation of five mutants low height of plantain hybrid ‘FHIA-21’ (<em>Musa </em>AAAB) by artificial inoculation of <em>Mycosphaerella fijiensis</em>

• Main Authors: Idalmis Bermúdez-Caraballoso, Michel Leiva-Mora, Yelenys Alvarado-Capó, Lourdes R. García, Novisel Veitía, Mayra Acosta-Suárez
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2007-01-01
• Series: Biotecnología Vegetal
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/351

The development of methodologies that allow to evaluate great volumes of plants and to detect in an early and fast way the searched characters facilitates greatly the works of selection in the Breeding Programs of banana and plantains. Five mutants of plantain hybrid ‘FHIA-21’ (Musa AAAB), treated with physical mutagenic agents (Gamma radiations, 60Co source), those that had been selected previously in the field by their low height were used for the studies. ‘Grande naine’ (Musa AAA) susceptible, ‘FHIA-20’ (Musa AAAB) as resistant and ‘FHIA- 21’ without irradiation were used as controls . They were multiplied in vitro and acclimatized for (60 days). The plants were inoculated with mycelia suspensions of Mycosphaerella fijiensis at a concentration of 1x 105. ml-1 by means of a brush by abaxial leaf surface of the four first open leaves. Affectation degrees caused by the pathogen were evaluated after 15, 30, 45 and 60 days plants had been inoculation. The first sympthoms appeared in the cultivar ‘Grande naine’ to the 45 days plants had been inoculated . Mutant ‘IBP 14-23’ stood out with the lowest values of affectation to the damages caused by Mycosphaerella fijiensis, when compared to the rest of mutants studied, corroborating its behavior in field. Key words: Black Sigatoka, earlier detection, In vitro mutagenesis