Research Article Molecular Diversity Among Sesame Varieties Of Tamil Nadu

• Main Author: Anitha, B.K., N. Manivannan, P. Vindhiya varman, C. Gopalakrishnan and K. Ganesamurthy
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2010-07-01
• Series: Electronic Journal of Plant Breeding
• Subjects: Genetic diversity; ISSR markers; Marker Aided Selection; PIC value
• Online Access: http://sites.google.com/site/ejpb10/vol-1-4-3/Vol-1-4-447-452.pdf?attredirects=0

Fourteen Inter Simple Sequence Repeat (ISSR) markers were used to estimate the molecular genetic diversityamong 10 sesame varieties cultivated in Tamil Nadu. The 14 ISSR primers in 10 sesame genotypes yielded 66reproducible amplified bands. The number of amplified bands varied from 1 to 7. Out of 66 bands, 65 werepolymorphic (98.5%). Average number of bands and polymorphic bands per primer were 4.7 and 4.6 respectively.The polymorphic information content (PIC) value ranged between 0.50 and 0.85. NTSYS-pc (ver 2.02)software was used to calculate the Jaccard’s similarity coefficients. The maximum similarity was observedbetween VRI 1 and VRI 2 followed by Paiyur 1 and TMV 6 and less similarity between VRI 1 and SVPR 1.Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis grouped the 10 genotypesin to five groups at 0.60 similarity coefficient. Varieties CO 1, SVPR 1 and TMV 7 were clustered in to threeseparate clusters indicating most divergent among the released varieties of Tamil Nadu.