Biosensors in Monitoring Water Quality and Safety: An Example of a Miniaturizable Whole-Cell Based Sensor for Hg²⁺ Optical Detection in Water

• Main Authors: Emanuele Luigi Sciuto, Maria Anna Coniglio, Domenico Corso, Jan Roelof van der Meer, Fabio Acerbi, Alberto Gola, Sebania Libertino
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: Water
• Subjects: water analysis; heavy metal sensing; mercury reporter; optical biosensor
• Online Access: https://www.mdpi.com/2073-4441/11/10/1986

Inorganic mercury (Hg²⁺) pollution of water reserves, especially drinking water, is an important issue in the environmental and public health field. Mercury is reported to be one of the most dangerous elements in nature since its accumulation and ingestion can lead to a series of permanent human diseases, affecting the kidneys and central nervous system. All the conventional approaches for assaying Hg²⁺ have some limitations in terms of bulky instruments and the cost and time required for the analysis. Here, we describe a miniaturizable and high-throughput bioluminescence sensor for Hg²⁺ detection in water, which combines the specificity of a living bacterial Hg²⁺ reporter cell, used as sensing element, with the performance of a silicon photomultiplier, used as optical detector. The proposed system paves the basis for portable analysis and low reactants consumption. The aim of the work is to propose a sensing strategy for total inorganic mercury evaluation in water. The proposed system can lay the basis for further studies and validations in order to develop rapid and portable technology that can be used in situ providing remote monitoring.