Analysis of clinical significance of quantitative expression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue

• Main Author: XI Pengcheng
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Clinical Hepatology 2014-08-01
• Series: Linchuang Gandanbing Zazhi
• Subjects: pancreatic neoplasms; vascular endothelial growth factor A; urinary plasminogen activator; gene expression
• Online Access: http://www.lcgdbzz.org/qk_content.asp?id=5926&ClassID=62613243

ObjectiveTo investigate the clinical significance of quantitative expression of vascular endothelial growth factor (VEGF) mRNA and urokinase-type plasminogen activator (uPA) mRNA in pancreatic cancer tissue. MethodsA retrospective study was conducted on the complete data of 30 patients with a pathological diagnosis of duct adenocarcinoma who were selected from those treated by radical resection of pancreatic head carcinoma from January 2008 to December 2011. Real-time quantitative PCR was used to measure the quantitative expression of VEGF mRNA and uPA mRNA in the pancreatic cancer tissues of the 30 cases and the normal pancreatic tissues of 6 controls, and its relationship with clinicopathological factors was analyzed. ResultsThe quantitative expression of VEGF mRNA and uPA mRNA was correlated with the histological differentiation and perineural invasion of pancreatic cancer. The quantitative expression of VEGF mRNA was significantly higher in patients with lymphatic metastasis than in those without lymphatic metastasis group (t=20.007, P=0.000). The quantitative expression of uPA mRNA was significantly lower in the tumors with a diameter of ≤2 cm than in the tumors with a diameter of ＞2 cm (t=7.539, P=0.000). Patients with duodenal invasion had a significantly higher quantitative expression of uPA mRNA than those without duodenal invasion (t=-2.089, P=0.037). The quantitative expression of uPA mRNA in stage III tumor tissues was significantly higher than that of uPA mRNA in stage I and II tumor tissues (t=-9.450, P=0.000). There was a positive correlation between VEGF mRNA expression and uPA mRNA expression (r=0.334, P=0.000). ConclusionThe overexpression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue may create an environment that enables the invasion by pancreatic cancer cells.