A Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases

A Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases

Vanillyl alcohol oxidase (VAO) and eugenol oxidase (EUGO) are flavin-dependent enzymes that catalyse the oxidation of para-substituted phenols. This makes them potentially interesting biocatalysts for the conversion of lignin-derived aromatic monomers to value-added compounds. To facilitate their bi...

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Bibliographic Details
Main Authors: Tom A. Ewing, Aster van Noord, Caroline E. Paul, Willem J. H. van Berkel
Format: Article
Language:English
Published: MDPI AG 2018-01-01
Series:Molecules
Subjects:
enzyme kinetics
flavoprotein
oxidase
screening assay
substrate specificity
Online Access:http://www.mdpi.com/1420-3049/23/1/164

Internet

http://www.mdpi.com/1420-3049/23/1/164

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