Lower affinity T cells are critical components and active participants of the immune response

Kinetic and biophysical parameters of T cell receptor (TCR) and peptide:MHC (pMHC) interaction define intrinsic factors required for T cell activation and differentiation. Although receptor ligand kinetics are somewhat cumbersome to assess experimentally, TCR:pMHC affinity has been shown to predict...

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Main Authors: Ryan eMartinez, Brian eEvavold
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-09-01
Series:Frontiers in Immunology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00468/full
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spelling doaj-b464dffc926e40728d159a4e7557968a2020-11-24T20:44:58ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-09-01610.3389/fimmu.2015.00468159611Lower affinity T cells are critical components and active participants of the immune responseRyan eMartinez0Brian eEvavold1Emory UniversityEmory UniversityKinetic and biophysical parameters of T cell receptor (TCR) and peptide:MHC (pMHC) interaction define intrinsic factors required for T cell activation and differentiation. Although receptor ligand kinetics are somewhat cumbersome to assess experimentally, TCR:pMHC affinity has been shown to predict peripheral T cell functionality and potential for forming memory. Multimeric forms of pMHC monomers have often been used to provide an indirect readout of higher affinity T cells due to their availability and ease of use while allowing simultaneous definition of other functional and phenotypic characteristics. However, multimeric pMHC reagents have introduced a bias that underestimates the lower affinity components contained in the highly diverse TCR repertoires of all polyclonal T cell responses. Advances in the identification of lower affinity cells have led to the examination of these cells and their contribution to the immune response. In this review we discuss the identification of high- versus low-affinity T cells as well as their attributed signaling and functional differences. Lastly mechanisms are discussed that maintain a diverse range of low- and high-affinity T cells.http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00468/fullT cellsTetramers2D assaysTCR AffinityT cell diversity
collection DOAJ
language English
format Article
sources DOAJ
author Ryan eMartinez
Brian eEvavold
spellingShingle Ryan eMartinez
Brian eEvavold
Lower affinity T cells are critical components and active participants of the immune response
Frontiers in Immunology
T cells
Tetramers
2D assays
TCR Affinity
T cell diversity
author_facet Ryan eMartinez
Brian eEvavold
author_sort Ryan eMartinez
title Lower affinity T cells are critical components and active participants of the immune response
title_short Lower affinity T cells are critical components and active participants of the immune response
title_full Lower affinity T cells are critical components and active participants of the immune response
title_fullStr Lower affinity T cells are critical components and active participants of the immune response
title_full_unstemmed Lower affinity T cells are critical components and active participants of the immune response
title_sort lower affinity t cells are critical components and active participants of the immune response
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2015-09-01
description Kinetic and biophysical parameters of T cell receptor (TCR) and peptide:MHC (pMHC) interaction define intrinsic factors required for T cell activation and differentiation. Although receptor ligand kinetics are somewhat cumbersome to assess experimentally, TCR:pMHC affinity has been shown to predict peripheral T cell functionality and potential for forming memory. Multimeric forms of pMHC monomers have often been used to provide an indirect readout of higher affinity T cells due to their availability and ease of use while allowing simultaneous definition of other functional and phenotypic characteristics. However, multimeric pMHC reagents have introduced a bias that underestimates the lower affinity components contained in the highly diverse TCR repertoires of all polyclonal T cell responses. Advances in the identification of lower affinity cells have led to the examination of these cells and their contribution to the immune response. In this review we discuss the identification of high- versus low-affinity T cells as well as their attributed signaling and functional differences. Lastly mechanisms are discussed that maintain a diverse range of low- and high-affinity T cells.
topic T cells
Tetramers
2D assays
TCR Affinity
T cell diversity
url http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00468/full
work_keys_str_mv AT ryanemartinez loweraffinitytcellsarecriticalcomponentsandactiveparticipantsoftheimmuneresponse
AT brianeevavold loweraffinitytcellsarecriticalcomponentsandactiveparticipantsoftheimmuneresponse
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