Enhancing the 1-Aminocyclopropane-1-Carboxylate Metabolic Rate of <i>Pseudomonas</i> sp. UW4 Intensifies Chemotactic Rhizocompetence

• Main Authors: Xiyang Gao, Tao Li, Wenliang Liu, Yan Zhang, Di Shang, Yuqian Gao, Yuancheng Qi, Liyou Qiu
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: Microorganisms
• Subjects: 1-aminocyclopropane-1-carboxylic acid deaminase; chemoattractant; chemotaxis; metabolic rate; plant growth promotion; <i>pseudomonas</i> sp.; rhizocompetence; rhizosphere colonization
• Online Access: https://www.mdpi.com/2076-2607/8/1/71

1-aminocyclopropane-1-carboxylic acid (ACC) is a strong metabolism-dependent chemoattractant for the plant beneficial rhizobacterium <i>Pseudomonas</i> sp. UW4. It is unknown whether enhancing the metabolic rate of ACC can intensify the chemotaxis activity towards ACC and rhizocompetence. In this study, we selected four promoters to transcribe the UW4 ACC deaminase (AcdS) gene in the UW4 &#916;<i>AcdS</i> mutant. PA is the UW4 <i>AcdS</i> gene promoter, PB20, PB10 and PB1 are synthetic promoters. The order of the <i>AcdS</i> gene expression level and AcdS activity of the four strains harboring the promoters were PB20 &gt; PA &gt; PB10 &gt; PB1. Interestingly, the AcdS activity of the four strains and their parent strain UW4 was significantly positively correlated with their chemotactic activity towards ACC, rhizosphere colonization, roots elongation and dry weight promotion. The results released that enhancing the AcdS activity of PGPRenable them to achieve strong chemotactic responses to ACC, rhizocompetence and plant growth promotion.