5′ Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice

5′ Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice

Using 5′ rapid amplification of cDNA ends, Illumina MiSeq, and basic flow cytometry, we systematically analyzed the expressed B cell receptor (BCR) repertoire in 14 healthy adult PBMCs, 5 HIV-1+ adult PBMCs, 5 cord blood samples, and 3 HIS-CD4/B mice, examining the full-length variable region of μ,...

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Bibliographic Details
Main Authors: Eric Waltari, Manxue Jia, Caroline S. Jiang, Hong Lu, Jing Huang, Cristina Fernandez, Andrés Finzi, Daniel E. Kaufmann, Martin Markowitz, Moriya Tsuji, Xueling Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-03-01
Series:Frontiers in Immunology
Subjects:
B cell repertoire
deep sequencing
HIV
cord blood
humanized mouse
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00628/full

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