Generation of an INSULIN-H2B-Cherry reporter human iPSC line
Differentiating human induced pluripotent stem cells (hiPSCs) into insulin (INS)-producing β-like cells has potential for diabetes research and therapy. Here, we generated a heterozygous fluorescent hiPSC reporter, labeling INS-producing β-like cells. We used CRISPR/Cas9 technology to knock-in a T2A...
Main Authors: | Anna Karolina Blöchinger, Johanna Siehler, Katharina Wißmiller, Alireza Shahryari, Ingo Burtscher, Heiko Lickert |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-05-01
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Series: | Stem Cell Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120301008 |
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