A protocol for rapid monocyte isolation and generation of singular human monocyte-derived dendritic cells.

A protocol for rapid monocyte isolation and generation of singular human monocyte-derived dendritic cells.

The monocyte-derived dendritic cells (moDCs) are a subset of dendritic cells widely used in immunological studies as a convenient and easy approach after isolation of mononuclear cells directly from peripheral blood mononuclear cells (PBMC). Both the purification and cell culture of monocytes impact...

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Bibliographic Details
Main Authors: Thaize Quiroga Chometon, Mariana da Silva Siqueira, Julie Carmo Sant Anna, Matheus Rogério Almeida, Mariana Gandini, Ana Cristina Martins de Almeida Nogueira, Paulo Renato Zuquim Antas
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0231132

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