A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations

Background: Increasing evidence points to a negative correlation between K-ras mutations and patient’s response to, or survival benefit after, treatment with EGFR-inhibitors. Therefore, rapid and reliable assays for mutational analysis of the K-ras gene are strongly needed.

Bibliographic Details
Main Authors:	D. Kramer, F. B. Thunnissen, M. I. Gallegos-Ruiz, E. F. Smit, P. E. Postmus, C. J. L. M. Meijer, P.J.F. Snijders, D. A. M. Heideman
Format:	Article
Language:	English
Published:	Hindawi Limited 2009-01-01
Series:	Cellular Oncology
Online Access:	http://dx.doi.org/10.3233/CLO-2009-0466

id	doaj-b3dca800acbe4d7e9877d48b2e718323
record_format	Article
spelling	doaj-b3dca800acbe4d7e9877d48b2e7183232020-11-25T00:06:42ZengHindawi LimitedCellular Oncology1570-58701875-86062009-01-0131316116710.3233/CLO-2009-0466A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras MutationsD. Kramer0F. B. Thunnissen1M. I. Gallegos-Ruiz2E. F. Smit3P. E. Postmus4C. J. L. M. Meijer5P.J.F. Snijders6D. A. M. Heideman7Department of Pathology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pathology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Medical Oncology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pulmonary Diseases, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pulmonary Diseases, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pathology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pathology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Pathology, VU University Medical Center, Amsterdam, The NetherlandsBackground: Increasing evidence points to a negative correlation between K-ras mutations and patient’s response to, or survival benefit after, treatment with EGFR-inhibitors. Therefore, rapid and reliable assays for mutational analysis of the K-ras gene are strongly needed.http://dx.doi.org/10.3233/CLO-2009-0466
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	D. Kramer F. B. Thunnissen M. I. Gallegos-Ruiz E. F. Smit P. E. Postmus C. J. L. M. Meijer P.J.F. Snijders D. A. M. Heideman
spellingShingle	D. Kramer F. B. Thunnissen M. I. Gallegos-Ruiz E. F. Smit P. E. Postmus C. J. L. M. Meijer P.J.F. Snijders D. A. M. Heideman A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations Cellular Oncology
author_facet	D. Kramer F. B. Thunnissen M. I. Gallegos-Ruiz E. F. Smit P. E. Postmus C. J. L. M. Meijer P.J.F. Snijders D. A. M. Heideman
author_sort	D. Kramer
title	A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
title_short	A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
title_full	A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
title_fullStr	A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
title_full_unstemmed	A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
title_sort	fast, sensitive and accurate high resolution melting (hrm) technology-based assay to screen for common k-ras mutations
publisher	Hindawi Limited
series	Cellular Oncology
issn	1570-5870 1875-8606
publishDate	2009-01-01
description	Background: Increasing evidence points to a negative correlation between K-ras mutations and patient’s response to, or survival benefit after, treatment with EGFR-inhibitors. Therefore, rapid and reliable assays for mutational analysis of the K-ras gene are strongly needed.
url	http://dx.doi.org/10.3233/CLO-2009-0466
work_keys_str_mv	AT dkramer afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT fbthunnissen afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT migallegosruiz afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT efsmit afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT pepostmus afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT cjlmmeijer afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT pjfsnijders afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT damheideman afastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT dkramer fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT fbthunnissen fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT migallegosruiz fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT efsmit fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT pepostmus fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT cjlmmeijer fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT pjfsnijders fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations AT damheideman fastsensitiveandaccuratehighresolutionmeltinghrmtechnologybasedassaytoscreenforcommonkrasmutations
_version_	1725420894213373952

A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations

Similar Items