Hypoglycemic, hypolipidemic and antioxidant effects of pioglitazone, insulin and synbiotic in diabetic rats

• Main Authors: K. Kavitha, A. Gopala Reddy, K. Kondal Reddy, C. S. V. Satish Kumar, G. Boobalan, K. Jayakanth
• Format: Article
• Language: English
• Published: Veterinary World 2016-02-01
• Series: Veterinary World
• Subjects: diabetes; insulin; oxidative stress; pioglitazone; synbiotic
• Online Access: http://www.veterinaryworld.org/Vol.9/February-2016/2.pdf
• ISSN: 0972-8988; 2231-0916

Aim: The objective of the study was to assess the effect of combination treatment of insulin, pioglitazone and synbiotic on streptozotocin (STZ)-induced diabetic rats. Materials and Methods: Diabetes mellitus was induced chemically by intraperitoneal administration of STZ (40 mg/kg b.wt) to male Sprague-Dawley rats. The rats were divided randomly into six groups of six rats in each. Group 1 was maintained as a normal control. Group 2 was maintained as diabetic control; Group 3 was treated with insulin; Group 4 with insulin + synbiotic; Group 5 with insulin + pioglitazone; and Group 6 with insulin + synbiotic + pioglitazone. All the animals were treated for 60 days. Results: Body weights, and concentration of reduced glutathione (GSH), and high-density lipoproteins cholesterol were significantly (p<0.05) reduced, whereas the concentration of blood glucose, total cholesterol, triglycerides, protein carbonyls and thiobarbituric acid reacting substances, and the activity of GSH peroxidase were significantly (p<0.05) elevated in Group 2 at the end of 8th week as compared to Group 1. The treatment Groups 3, 4, 5 and 6 revealed improvement in all the parameters, and the highest improvement was observed in combination Group 6. Conclusion: From this study, it is concluded that combination of insulin, pioglitazone and synbiotic is useful in treating diabetes.