Derivation of human embryonic stem cell line Genea019

Derivation of human embryonic stem cell line Genea019

The Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CA...

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Main Authors: Biljana Dumevska, Teija Peura, Robert McKernan, Divya Goel, Uli Schmidt
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000507
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spelling doaj-b3889ac27bcb4920abdeaf917e34475a2020-11-25T00:40:26ZengElsevierStem Cell Research1873-50611876-77532016-03-0116239740010.1016/j.scr.2016.02.008Derivation of human embryonic stem cell line Genea019Biljana DumevskaTeija PeuraRobert McKernanDivya GoelUli SchmidtThe Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CAG repeat length, compared to HD affected sibling Genea020. Pluripotency of Genea019 was demonstrated with 75% of cells expressing Nanog, 89% Oct4, 48% Tra1-60 and 85% SSEA4, a Pluritest Pluripotency score of 22.97, Novelty score of 1.42, tri-lineage teratoma formation and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.http://www.sciencedirect.com/science/article/pii/S1873506116000507
collection DOAJ
language English
format Article
sources DOAJ
author Biljana Dumevska
Teija Peura
Robert McKernan
Divya Goel
Uli Schmidt
spellingShingle Biljana Dumevska
Teija Peura
Robert McKernan
Divya Goel
Uli Schmidt
Derivation of human embryonic stem cell line Genea019
Stem Cell Research
author_facet Biljana Dumevska
Teija Peura
Robert McKernan
Divya Goel
Uli Schmidt
author_sort Biljana Dumevska
title Derivation of human embryonic stem cell line Genea019
title_short Derivation of human embryonic stem cell line Genea019
title_full Derivation of human embryonic stem cell line Genea019
title_fullStr Derivation of human embryonic stem cell line Genea019
title_full_unstemmed Derivation of human embryonic stem cell line Genea019
title_sort derivation of human embryonic stem cell line genea019
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CAG repeat length, compared to HD affected sibling Genea020. Pluripotency of Genea019 was demonstrated with 75% of cells expressing Nanog, 89% Oct4, 48% Tra1-60 and 85% SSEA4, a Pluritest Pluripotency score of 22.97, Novelty score of 1.42, tri-lineage teratoma formation and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.
url http://www.sciencedirect.com/science/article/pii/S1873506116000507
work_keys_str_mv AT biljanadumevska derivationofhumanembryonicstemcelllinegenea019
AT teijapeura derivationofhumanembryonicstemcelllinegenea019
AT robertmckernan derivationofhumanembryonicstemcelllinegenea019
AT divyagoel derivationofhumanembryonicstemcelllinegenea019
AT ulischmidt derivationofhumanembryonicstemcelllinegenea019
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