Short-term ex-vivo exposure to hydrogen sulfide enhances murine hematopoietic stem and progenitor cell migration, homing, and proliferation

• Main Authors: Anoushka Khanna, Namita Indracanti, Rina Chakrabarti, Prem Kumar Indraganti
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2020-01-01
• Series: Cell Adhesion & Migration
• Subjects: stem cell migration; homing; bone marrow transplantation; mitochondrial function; cxcr4 expression
• Online Access: http://dx.doi.org/10.1080/19336918.2020.1842131

For successful transplantation of Hematopoietic Stem cells (HSCs), it is quite necessary that efficient homing, engraftment and retention of HSC self-renewal capacity takes place, which is often restricted due to inadequate number of adult HSCs. Here, we report that short-term ex-vivo treatment of mouse bone marrow mononuclear cells (BMMNCs) to Sodium Hydrogen Sulfide (NaHS, hydrogen sulfide-H2S donor) can be used as a possible strategy to overcome such hurdle. H2S increases the expression of CXCR4 on HSPCs, enhancing their migration toward SDF-1α in-vitro and thus homing to BM niche. . Additionally, in-vitro studies revealed that H2S has a role in activating mitochondria, thus, pushing quiescent HSCs into division. These results suggest a readily available and cost-effective method to facilitate efficient HSC transplantation.