Exploring the Role of L10 Loop in New Delhi Metallo-β-lactamase (NDM-1): Kinetic and Dynamic Studies

Exploring the Role of L10 Loop in New Delhi Metallo-β-lactamase (NDM-1): Kinetic and Dynamic Studies

Four NDM-1 mutants (L218T, L221T, L269H and L221T/Y229W) were generated in order to investigate the role of leucines positioned in L10 loop. A detailed kinetic analysis stated that these amino acid substitutions modified the hydrolytic profile of NDM-1 against some β-lactams. Significant reduction o...

Full description

Bibliographic Details
Main Authors: Alessandra Piccirilli, Emanuele Criscuolo, Fabrizia Brisdelli, Paola Sandra Mercuri, Sabrina Cherubini, Maria Laura De Sciscio, Mauro Maccarrone, Moreno Galleni, Gianfranco Amicosante, Mariagrazia Perilli
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Molecules
Subjects:
metallo-β-lactamases
NDM-1
kinetic studies
molecular dynamic
Online Access:https://www.mdpi.com/1420-3049/26/18/5489

Internet

https://www.mdpi.com/1420-3049/26/18/5489

Similar Items