Olfactory receptor 78 modulates renin but not baseline blood pressure

Abstract Olfactory receptor 78 (Olfr78) is a G protein‐coupled receptor (GPCR) that is expressed in the juxtaglomerular apparatus (JGA) of the kidney as well as the peripheral vasculature, and is activated by gut microbial metabolites. We previously reported that Olfr78 plays a role in renin secreti...

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Main Authors: Brian G. Poll, Jiaojiao Xu, Kunal Gupta, Tyler B. Shubitowski, Jennifer L. Pluznick
Format: Article
Language:English
Published: Wiley 2021-09-01
Series:Physiological Reports
Subjects:
Online Access:https://doi.org/10.14814/phy2.15017
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spelling doaj-b353768995f4435f8e69fffe4aa19f2d2021-09-28T14:45:32ZengWileyPhysiological Reports2051-817X2021-09-01918n/an/a10.14814/phy2.15017Olfactory receptor 78 modulates renin but not baseline blood pressureBrian G. Poll0Jiaojiao Xu1Kunal Gupta2Tyler B. Shubitowski3Jennifer L. Pluznick4Department of Physiology Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Physiology Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Physiology Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Physiology Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Physiology Johns Hopkins University School of Medicine Baltimore Maryland USAAbstract Olfactory receptor 78 (Olfr78) is a G protein‐coupled receptor (GPCR) that is expressed in the juxtaglomerular apparatus (JGA) of the kidney as well as the peripheral vasculature, and is activated by gut microbial metabolites. We previously reported that Olfr78 plays a role in renin secretion in isolated glomeruli, and that Olfr78 knockout (KO) mice have lower plasma renin activity. We also noted that in anesthetized mice, Olfr78KO appeared to be hypotensive. In this study, we used radiotelemetry to determine the role of Olfr78 in chronic blood pressure regulation. We found that the blood pressure of Olfr78KO mice is not significantly different than that of their WT counterparts at baseline, or on high‐ or low‐salt diets. However, Olfr78KO mice have depressed heart rates on high‐salt diets. We also report that Olfr78KO mice have lower renin protein levels associated with glomeruli. Finally, we developed a mouse where Olfr78 was selectively knocked out in the JGA, which phenocopied the lower renin association findings. In sum, these experiments suggest that Olfr78 modulates renin, but does not play an active role in blood pressure regulation at baseline, and is more likely activated by high levels of short chain fatty acids or hypotensive events. This study provides important context to our knowledge of Olfr78 in BP regulation.https://doi.org/10.14814/phy2.15017blood pressureolfactory receptorsrenin
collection DOAJ
language English
format Article
sources DOAJ
author Brian G. Poll
Jiaojiao Xu
Kunal Gupta
Tyler B. Shubitowski
Jennifer L. Pluznick
spellingShingle Brian G. Poll
Jiaojiao Xu
Kunal Gupta
Tyler B. Shubitowski
Jennifer L. Pluznick
Olfactory receptor 78 modulates renin but not baseline blood pressure
Physiological Reports
blood pressure
olfactory receptors
renin
author_facet Brian G. Poll
Jiaojiao Xu
Kunal Gupta
Tyler B. Shubitowski
Jennifer L. Pluznick
author_sort Brian G. Poll
title Olfactory receptor 78 modulates renin but not baseline blood pressure
title_short Olfactory receptor 78 modulates renin but not baseline blood pressure
title_full Olfactory receptor 78 modulates renin but not baseline blood pressure
title_fullStr Olfactory receptor 78 modulates renin but not baseline blood pressure
title_full_unstemmed Olfactory receptor 78 modulates renin but not baseline blood pressure
title_sort olfactory receptor 78 modulates renin but not baseline blood pressure
publisher Wiley
series Physiological Reports
issn 2051-817X
publishDate 2021-09-01
description Abstract Olfactory receptor 78 (Olfr78) is a G protein‐coupled receptor (GPCR) that is expressed in the juxtaglomerular apparatus (JGA) of the kidney as well as the peripheral vasculature, and is activated by gut microbial metabolites. We previously reported that Olfr78 plays a role in renin secretion in isolated glomeruli, and that Olfr78 knockout (KO) mice have lower plasma renin activity. We also noted that in anesthetized mice, Olfr78KO appeared to be hypotensive. In this study, we used radiotelemetry to determine the role of Olfr78 in chronic blood pressure regulation. We found that the blood pressure of Olfr78KO mice is not significantly different than that of their WT counterparts at baseline, or on high‐ or low‐salt diets. However, Olfr78KO mice have depressed heart rates on high‐salt diets. We also report that Olfr78KO mice have lower renin protein levels associated with glomeruli. Finally, we developed a mouse where Olfr78 was selectively knocked out in the JGA, which phenocopied the lower renin association findings. In sum, these experiments suggest that Olfr78 modulates renin, but does not play an active role in blood pressure regulation at baseline, and is more likely activated by high levels of short chain fatty acids or hypotensive events. This study provides important context to our knowledge of Olfr78 in BP regulation.
topic blood pressure
olfactory receptors
renin
url https://doi.org/10.14814/phy2.15017
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