Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tol...

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Main Authors: Lun Yao, Kiyan Shabestary, Sara M. Björk, Johannes Asplund-Samuelsson, Haakan N. Joensson, Michael Jahn, Elton P. Hudson
Format: Article
Language:English
Published: Nature Publishing Group 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15491-7
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spelling doaj-b347e4caadcb4489961c56c34a9c1a762021-05-11T08:55:14ZengNature Publishing GroupNature Communications2041-17232020-04-0111111310.1038/s41467-020-15491-7Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypesLun Yao0Kiyan Shabestary1Sara M. Björk2Johannes Asplund-Samuelsson3Haakan N. Joensson4Michael Jahn5Elton P. Hudson6Science for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyScience for Life Laboratory, KTH - Royal Institute of TechnologyDeveloping cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tolerance and production.https://doi.org/10.1038/s41467-020-15491-7
collection DOAJ
language English
format Article
sources DOAJ
author Lun Yao
Kiyan Shabestary
Sara M. Björk
Johannes Asplund-Samuelsson
Haakan N. Joensson
Michael Jahn
Elton P. Hudson
spellingShingle Lun Yao
Kiyan Shabestary
Sara M. Björk
Johannes Asplund-Samuelsson
Haakan N. Joensson
Michael Jahn
Elton P. Hudson
Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
Nature Communications
author_facet Lun Yao
Kiyan Shabestary
Sara M. Björk
Johannes Asplund-Samuelsson
Haakan N. Joensson
Michael Jahn
Elton P. Hudson
author_sort Lun Yao
title Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_short Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_full Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_fullStr Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_full_unstemmed Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_sort pooled crispri screening of the cyanobacterium synechocystis sp pcc 6803 for enhanced industrial phenotypes
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-04-01
description Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tolerance and production.
url https://doi.org/10.1038/s41467-020-15491-7
work_keys_str_mv AT lunyao pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT kiyanshabestary pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT sarambjork pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT johannesasplundsamuelsson pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT haakannjoensson pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT michaeljahn pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
AT eltonphudson pooledcrispriscreeningofthecyanobacteriumsynechocystissppcc6803forenhancedindustrialphenotypes
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