Partially Hydrolyzed Guar Gum Attenuates <span style="font-variant: small-caps">d</span>-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats

• Main Authors: Xiaoyan Liu, Chenxuan Wu, Dong Han, Jun Liu, Haijie Liu, Zhengqiang Jiang
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: International Journal of Molecular Sciences
• Subjects: partially hydrolyzed guar gum; <span style="font-variant: small-caps">d</span>-galactose; aging; oxidative damage; dysbacteriosis
• Online Access: https://www.mdpi.com/1422-0067/20/19/4861

Partially hydrolyzed guar gum (PHGG) has received considerable attention for its various bioactive functions. The injection of <span style="font-variant: small-caps;">d</span>-galactose can cause aging-related injury which is usually resulted from oxidative stress on tissues and cells. In this study, <span style="font-variant: small-caps;">d</span>-galactose (200 mg/kg/day) was injected into rats, and the protective effects of PHGG (500, 1000, and 1500 mg/kg/day) against oxidative damages, as well as its probiotic functions, were analyzed. The results showed that PHGG treatment at a concentration of 1500 mg/kg/day greatly reduced the levels of lactic acid, nitric oxide, inducible nitric oxide synthase, advanced glycation end products, and increased the telomerase activity, by 7.60%, 9.25%, 12.28%, 14.58%, and 9.01%, respectively. Moreover, PHGG significantly elevated the activities of antioxidant enzymes and decreased the content of malondialdehyde in rat serum and brain. The oxidative damage was also significantly alleviated in the liver and hippocampus and the expressions of brain-derived neurotrophic factor and choline acetyltransferase also increased. Furthermore, PHGG treatment could significantly regulated the expression of sirtuin 1, forkhead box O1, and tumor protein p53 in the hippocampus. It also increased the levels of organic acids and improved the composition of intestinal microbiota. These findings demonstrated that PHGG treatment could effectively alleviate the oxidative damage and dysbacteriosis.