Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids

Cancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (C...

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Main Authors: Francesco Amato, Colin Rae, Maria Giuseppina Prete, Chiara Braconi
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:Cells
Subjects:
CCA
PDO
Online Access:https://www.mdpi.com/2073-4409/9/4/832
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spelling doaj-b2ebd6fc322a4573962a972b3f6fd2952020-11-25T02:05:23ZengMDPI AGCells2073-44092020-03-01983283210.3390/cells9040832Cholangiocarcinoma Disease Modelling Through Patients Derived OrganoidsFrancesco Amato0Colin Rae1Maria Giuseppina Prete2Chiara Braconi3Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKCancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (CCA) PDO is still sparse but growing. CCA PDO lines have been established from resected early stage disease, advanced cancers and highly chemorefractory tumours. Cancer PDO was shown to recapitulate the 3D morphology, genomic landscape and transcriptomic profile of the source counterpart. They proved to be a valued model for drug discovery and sensitivity testing, and they showed to mimic the drug response observed in vivo in the patients. However, PDO lack representation of the intratumour heterogeneity and the tumour-stroma interaction. The efficiency rate of CCA PDO within the three different subtypes, intrahepatic, perihilar and distal, is still to be explored. In this manuscript we will review evidence for CCA PDO highlighting advantages and limitations of this novel disease model.https://www.mdpi.com/2073-4409/9/4/832CCAorganoidPDOpersonalized medicinebiliary cancer
collection DOAJ
language English
format Article
sources DOAJ
author Francesco Amato
Colin Rae
Maria Giuseppina Prete
Chiara Braconi
spellingShingle Francesco Amato
Colin Rae
Maria Giuseppina Prete
Chiara Braconi
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
Cells
CCA
organoid
PDO
personalized medicine
biliary cancer
author_facet Francesco Amato
Colin Rae
Maria Giuseppina Prete
Chiara Braconi
author_sort Francesco Amato
title Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
title_short Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
title_full Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
title_fullStr Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
title_full_unstemmed Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
title_sort cholangiocarcinoma disease modelling through patients derived organoids
publisher MDPI AG
series Cells
issn 2073-4409
publishDate 2020-03-01
description Cancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (CCA) PDO is still sparse but growing. CCA PDO lines have been established from resected early stage disease, advanced cancers and highly chemorefractory tumours. Cancer PDO was shown to recapitulate the 3D morphology, genomic landscape and transcriptomic profile of the source counterpart. They proved to be a valued model for drug discovery and sensitivity testing, and they showed to mimic the drug response observed in vivo in the patients. However, PDO lack representation of the intratumour heterogeneity and the tumour-stroma interaction. The efficiency rate of CCA PDO within the three different subtypes, intrahepatic, perihilar and distal, is still to be explored. In this manuscript we will review evidence for CCA PDO highlighting advantages and limitations of this novel disease model.
topic CCA
organoid
PDO
personalized medicine
biliary cancer
url https://www.mdpi.com/2073-4409/9/4/832
work_keys_str_mv AT francescoamato cholangiocarcinomadiseasemodellingthroughpatientsderivedorganoids
AT colinrae cholangiocarcinomadiseasemodellingthroughpatientsderivedorganoids
AT mariagiuseppinaprete cholangiocarcinomadiseasemodellingthroughpatientsderivedorganoids
AT chiarabraconi cholangiocarcinomadiseasemodellingthroughpatientsderivedorganoids
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