Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids
Cancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (C...
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doaj-b2ebd6fc322a4573962a972b3f6fd2952020-11-25T02:05:23ZengMDPI AGCells2073-44092020-03-01983283210.3390/cells9040832Cholangiocarcinoma Disease Modelling Through Patients Derived OrganoidsFrancesco Amato0Colin Rae1Maria Giuseppina Prete2Chiara Braconi3Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKInstitute of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKCancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (CCA) PDO is still sparse but growing. CCA PDO lines have been established from resected early stage disease, advanced cancers and highly chemorefractory tumours. Cancer PDO was shown to recapitulate the 3D morphology, genomic landscape and transcriptomic profile of the source counterpart. They proved to be a valued model for drug discovery and sensitivity testing, and they showed to mimic the drug response observed in vivo in the patients. However, PDO lack representation of the intratumour heterogeneity and the tumour-stroma interaction. The efficiency rate of CCA PDO within the three different subtypes, intrahepatic, perihilar and distal, is still to be explored. In this manuscript we will review evidence for CCA PDO highlighting advantages and limitations of this novel disease model.https://www.mdpi.com/2073-4409/9/4/832CCAorganoidPDOpersonalized medicinebiliary cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesco Amato Colin Rae Maria Giuseppina Prete Chiara Braconi |
spellingShingle |
Francesco Amato Colin Rae Maria Giuseppina Prete Chiara Braconi Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids Cells CCA organoid PDO personalized medicine biliary cancer |
author_facet |
Francesco Amato Colin Rae Maria Giuseppina Prete Chiara Braconi |
author_sort |
Francesco Amato |
title |
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids |
title_short |
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids |
title_full |
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids |
title_fullStr |
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids |
title_full_unstemmed |
Cholangiocarcinoma Disease Modelling Through Patients Derived Organoids |
title_sort |
cholangiocarcinoma disease modelling through patients derived organoids |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-03-01 |
description |
Cancer organoids are 3D phenotypic cultures that can be established from resected or biopsy tumour samples and can be grown as mini tumours in the dish. Flourishing evidence supports the feasibility of patient derived organoids (PDO) from a number of solid tumours. Evidence for cholangiocarcinoma (CCA) PDO is still sparse but growing. CCA PDO lines have been established from resected early stage disease, advanced cancers and highly chemorefractory tumours. Cancer PDO was shown to recapitulate the 3D morphology, genomic landscape and transcriptomic profile of the source counterpart. They proved to be a valued model for drug discovery and sensitivity testing, and they showed to mimic the drug response observed in vivo in the patients. However, PDO lack representation of the intratumour heterogeneity and the tumour-stroma interaction. The efficiency rate of CCA PDO within the three different subtypes, intrahepatic, perihilar and distal, is still to be explored. In this manuscript we will review evidence for CCA PDO highlighting advantages and limitations of this novel disease model. |
topic |
CCA organoid PDO personalized medicine biliary cancer |
url |
https://www.mdpi.com/2073-4409/9/4/832 |
work_keys_str_mv |
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