Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids

Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids

We conducted systemic assessments on the toxicity of silicon dioxide (SiO<sub>2</sub>) and titanium dioxide (TiO<sub>2</sub>) nanoparticles using different forms of normal colon cells (CCD-18Co), in vivo and in human colon organoids. The in vivo acute oral toxicity data showe...

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Main Authors: Sung Bum Park, Won Hoon Jung, Ki Young Kim, Byumseok Koh
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Molecules
Subjects:
silicon dioxide nanoparticle
titanium dioxide nanoparticle
cytotoxicity
in vivo toxicity
colon organoids
Online Access:https://www.mdpi.com/1420-3049/25/16/3594
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spelling doaj-b2e9aa302e734a65934ccba3e6be7f612020-11-25T03:15:49ZengMDPI AGMolecules1420-30492020-08-01253594359410.3390/molecules25163594Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon OrganoidsSung Bum Park0Won Hoon Jung1Ki Young Kim2Byumseok Koh3Drug Discovery Platform Research Center, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, KoreaDrug Discovery Platform Research Center, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, KoreaDrug Discovery Platform Research Center, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, KoreaDrug Discovery Platform Research Center, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, KoreaWe conducted systemic assessments on the toxicity of silicon dioxide (SiO<sub>2</sub>) and titanium dioxide (TiO<sub>2</sub>) nanoparticles using different forms of normal colon cells (CCD-18Co), in vivo and in human colon organoids. The in vivo acute oral toxicity data showed that the LD<sub>50</sub> values are greater than 2000 mg/kg for both the SiO<sub>2</sub> and TiO<sub>2</sub> nanoparticles; however, the SiO<sub>2</sub> and TiO<sub>2</sub> nanoparticles induced cytotoxicity in two-dimensional CCD-18Co cells and three-dimensional CCD-18Co spheroids and human colon organoids, with IC<sub>50</sub> values of 0.6, 0.8 and 0.3 mM for SiO<sub>2</sub> and 2.5, 1.1 and 12.5 mM for TiO<sub>2</sub> nanoparticles, respectively. The data suggest that, when SiO<sub>2</sub> and TiO<sub>2</sub> are in nanoparticle form, cytotoxicity is induced; thus, care should be taken with these materials.https://www.mdpi.com/1420-3049/25/16/3594silicon dioxide nanoparticletitanium dioxide nanoparticlecytotoxicityin vivo toxicitycolon organoids
collection DOAJ
language English
format Article
sources DOAJ
author Sung Bum Park
Won Hoon Jung
Ki Young Kim
Byumseok Koh
spellingShingle Sung Bum Park
Won Hoon Jung
Ki Young Kim
Byumseok Koh
Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
Molecules
silicon dioxide nanoparticle
titanium dioxide nanoparticle
cytotoxicity
in vivo toxicity
colon organoids
author_facet Sung Bum Park
Won Hoon Jung
Ki Young Kim
Byumseok Koh
author_sort Sung Bum Park
title Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
title_short Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
title_full Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
title_fullStr Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
title_full_unstemmed Toxicity Assessment of SiO<sub>2</sub> and TiO<sub>2</sub> in Normal Colon Cells, In Vivo and in Human Colon Organoids
title_sort toxicity assessment of sio<sub>2</sub> and tio<sub>2</sub> in normal colon cells, in vivo and in human colon organoids
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2020-08-01
description We conducted systemic assessments on the toxicity of silicon dioxide (SiO<sub>2</sub>) and titanium dioxide (TiO<sub>2</sub>) nanoparticles using different forms of normal colon cells (CCD-18Co), in vivo and in human colon organoids. The in vivo acute oral toxicity data showed that the LD<sub>50</sub> values are greater than 2000 mg/kg for both the SiO<sub>2</sub> and TiO<sub>2</sub> nanoparticles; however, the SiO<sub>2</sub> and TiO<sub>2</sub> nanoparticles induced cytotoxicity in two-dimensional CCD-18Co cells and three-dimensional CCD-18Co spheroids and human colon organoids, with IC<sub>50</sub> values of 0.6, 0.8 and 0.3 mM for SiO<sub>2</sub> and 2.5, 1.1 and 12.5 mM for TiO<sub>2</sub> nanoparticles, respectively. The data suggest that, when SiO<sub>2</sub> and TiO<sub>2</sub> are in nanoparticle form, cytotoxicity is induced; thus, care should be taken with these materials.
topic silicon dioxide nanoparticle
titanium dioxide nanoparticle
cytotoxicity
in vivo toxicity
colon organoids
url https://www.mdpi.com/1420-3049/25/16/3594
work_keys_str_mv AT sungbumpark toxicityassessmentofsiosub2subandtiosub2subinnormalcoloncellsinvivoandinhumancolonorganoids
AT wonhoonjung toxicityassessmentofsiosub2subandtiosub2subinnormalcoloncellsinvivoandinhumancolonorganoids
AT kiyoungkim toxicityassessmentofsiosub2subandtiosub2subinnormalcoloncellsinvivoandinhumancolonorganoids
AT byumseokkoh toxicityassessmentofsiosub2subandtiosub2subinnormalcoloncellsinvivoandinhumancolonorganoids
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