Establishment of a new human osteosarcoma cell line, UTOS-1: cytogenetic characterization by array comparative genomic hybridization

• Main Authors: Oya Takeshi, Hori Takeshi, Nogami Shigeharu, Kanamori Masahiko, Yasuda Taketoshi, Suzuki Kayo, Kimura Tomoatsu
• Format: Article
• Language: English
• Published: BMC 2009-02-01
• Series: Journal of Experimental & Clinical Cancer Research
• Online Access: http://www.jeccr.com/content/28/1/26

<p>Abstract</p> <p>The cytogenetic characteristics of osteosarcoma (OS) remain controversial. The establishment of a new human OS cell line may improve the characterization. We report the establishment of a new human osteosarcoma cell line, UTOS-1, from a typical osteoblastic OS of an 18-year-old man. Cultured UTOS-1 cells are spindle-shaped, and have been maintained <it>in vitro </it>for over 50 passages in more than 2 years. Xenografted UTOS-1 cells exhibit features typical of OS, such as production of osteoid or immature bone matrix, and proliferation potency <it>in vivo</it>. UTOS-1 also exhibit morphological and immunohistochemical characteristics typical of osteoblastic OS. Chromosomal analysis by G-band show 73~85 chromosomes with complicated translocations. Array CGH show frequent gains at locus <it>DAB2 </it>at chromosome 5q13, <it>CCND2 </it>at 12p13, <it>MDM2 </it>at 12q14.3-q15, <it>FLI </it>and <it>TOP3A </it>at 17p11.2-p12 and <it>OCRL1 </it>at Xq25, and show frequent losses at <it>HTR1B </it>at 6q13, <it>D6S268 </it>at 6q16.3-q21, <it>SHGC17327 </it>at 18ptel, and <it>STK6 </it>at 20q13.2-q13.3. The UTOS-1 cell line may prove useful for biologic and molecular pathogenetic investigations of human OS.</p>