Isomangiferin Attenuates Renal Injury in Diabetic Mice via Inhibiting Inflammation

• Main Authors: Yue S, Xue N, Li H, Chen Z, Huang B, Wang X
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-11-01
• Series: Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy
• Subjects: isomangiferin; renal injury; inflammation; hmgb1/nlrp3/nf-κb; diabetics
• Online Access: https://www.dovepress.com/isomangiferin-attenuates-renal-injury-in-diabetic-mice-via-inhibiting--peer-reviewed-article-DMSO

Shuwen Yue,1,* Ning Xue,2,* Honglei Li,3 Zhen Chen,1 Baosheng Huang,4 Xing Wang1 1Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing, People’s Republic of China; 2Department of Acupuncture, Jurong Hospital Affiliated to Jiangsu University, Zhenjiang, People’s Republic of China; 3Department of Pharmacy, Kangda College of Nanjing Medical University, Lianyungang, People’s Republic of China; 4Department of Neurosurgery, Sir Run Run Hospital, Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xing WangChina Pharmaceutical University, Nanjing, Jiangsu 211199, People’s Republic of ChinaEmail [e-mail] HuangSir Run Run Hospital, Nanjing Medical University, No. 101, Longmiandadao, Jiangning District, Nanjing, Jiangsu 211166, People’s Republic of ChinaEmail [e-mail]: Renal injury induced by diabetes is reported to be associated with inflammation. Isomangiferin (ISO), a xanthone C-glucoside from the Cyclopia subfamily, exhibits many pharmacological properties. This study aimed to evaluate the protection of ISO against renal damage in diabetic mice.Methods: Serum glucose, insulin, uric acid, creatinine, total cholesterol (TC), triglyceride (TG), and inflammatory cytokines in serum and the kidney of db/db diabetes model mice were detected. The components of high mobility group protein B1 (HMGB1)/NACHT leucine-rich repeat- and PYD-containing 3 (NLRP3)/nuclear factor kappa-B (NF-κB) pathway in the kidney were detected by Western blot and immunohistochemical analysis.Results: ISO improved lipid profile and glucose tolerance, and inhibited the production of inflammatory cytokines in a db/db model mice. Moreover, ISO decreased biochemical indexes in the serum and inhibited the activation of HMGB1/NLRP3/NF-κB signaling in the kidney of db/db model mice.Conclusion: ISO provides protection against renal injury via inhibiting HMGB1/NLRP3/NF-κB signaling in a diabetic mouse model.Keywords: isomangiferin, renal injury, inflammation, HMGB1/NLRP3/NF-κB, diabetics