Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish ( Rhamdia quelen )

• Main Authors: Jane Mello Lopes, Carine de Freitas Souza, Bianca Schindler, Carlos Garrido Pinheiro, Joseânia Salbego, Jefferson Costa de Siqueira, Berta Maria Heinzmann, Bernardo Baldisserotto
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Ictiologia 2018-06-01
• Series: Neotropical Ichthyology
• Subjects: Anestesia; Fluxo de íons; Frequência ventilatória; Óleo de laranja; Óleo de limão
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200204&lng=en&tlng=en

ABSTRACT This study investigated the anesthetic effect of the essential oils (EOs) from the peel of Citrus x aurantium (EOCA) and Citrus x latifolia (EOCL) on silver catfish Rhamdia quelen. Fish were exposed to different concentrations of EOCA and EOCL to determine time of anesthesia induction and recovery. Induction of anesthesia was observed in all fish exposed to 400, 600 or 800 μL L−1 EOCA and 300, 400 or 500 μL L−1 EOCL. Another group of fish were exposed for 8 h to 50, 100, or 200 μL L−1 of either EOs. Overall, fish exposed to ethanol and both EOs presented higher ventilatory frequencies (VF) than the control group throughout the 8 h of exposure. Net ion (Na+, K+ and Cl−) effluxes and ammonia excretion were significantly lower in fish exposed to 50, 100 or 200 μL L−1 of either EOs compared to control fish. Mortality was 37% in fish exposed to 200 μL L−1 of either EOs after 8 h. These findings suggest that EOCA and EOCL are useful anesthetics and sedatives for Rhamdia quelen, but their usefulness as alternatives to reduce stress in fish transportation at the lower concentrations tested (50-100 µL L−1) deserves further study.