3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds

Carla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE – A.O. Ospedale Niguarda Ca' Granda, Milan; 3Laboratory of B...

Full description

Bibliographic Details
Main Authors: Cunha C, Panseri S, Villa O, Silva D, Gelain F
Format: Article
Language:English
Published: Dove Medical Press 2011-05-01
Series:International Journal of Nanomedicine
Online Access:http://www.dovepress.com/3d-culture-of-adult-mouse-neural-stem-cells-within-functionalized-self-a7313
id doaj-b2b0b21ea9b94b2e8bffc4cc9053e319
record_format Article
spelling doaj-b2b0b21ea9b94b2e8bffc4cc9053e3192020-11-24T23:04:37ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132011-05-012011default9439553D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffoldsCunha CPanseri SVilla OSilva DGelain FCarla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE – A.O. Ospedale Niguarda Ca' Granda, Milan; 3Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna; 4Laboratory of Nano-Biomagnetism, Institute of Science and Technology for Ceramics, National Research Council, Faenza, ItalyAbstract: Three-dimensional (3D) in vitro models of cell culture aim to fill the gap between the standard two-dimensional cell studies and the in vivo environment. Especially for neural tissue regeneration approaches where there is little regenerative capacity, these models are important for mimicking the extracellular matrix in providing support, allowing the natural flow of oxygen, nutrients, and growth factors, and possibly favoring neural cell regrowth. We have previously demonstrated that a new self-assembling nanostructured biomaterial, based on matrigel, was able to support adult neural stem cell (NSC) culture. In this study, we developed a new 3D cell culture system that takes advantage of the nano- and microfiber assembling process, under physiologic conditions, of these biomaterials. The assembled scaffold forms an intricate and biologically active matrix that displays specifically designed functional motifs: RGD (Arg-Gly-Asp), BMHP1 (bone marrow homing peptide 1), and BMHP2, for the culture of adult NSCs. These scaffolds were prepared at different concentrations, and microscopic examination of the cell-embedded scaffolds showed that NSCs are viable and they proliferate and differentiate within the nanostructured environment of the scaffold. Such a model has the potential to be tailored to develop ad hoc designed peptides for specific cell lines.Keywords: biomaterials, tissue engineering, 3D in vitro modelhttp://www.dovepress.com/3d-culture-of-adult-mouse-neural-stem-cells-within-functionalized-self-a7313
collection DOAJ
language English
format Article
sources DOAJ
author Cunha C
Panseri S
Villa O
Silva D
Gelain F
spellingShingle Cunha C
Panseri S
Villa O
Silva D
Gelain F
3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
International Journal of Nanomedicine
author_facet Cunha C
Panseri S
Villa O
Silva D
Gelain F
author_sort Cunha C
title 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_short 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_full 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_fullStr 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_full_unstemmed 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_sort 3d culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
publisher Dove Medical Press
series International Journal of Nanomedicine
issn 1176-9114
1178-2013
publishDate 2011-05-01
description Carla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE – A.O. Ospedale Niguarda Ca' Granda, Milan; 3Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna; 4Laboratory of Nano-Biomagnetism, Institute of Science and Technology for Ceramics, National Research Council, Faenza, ItalyAbstract: Three-dimensional (3D) in vitro models of cell culture aim to fill the gap between the standard two-dimensional cell studies and the in vivo environment. Especially for neural tissue regeneration approaches where there is little regenerative capacity, these models are important for mimicking the extracellular matrix in providing support, allowing the natural flow of oxygen, nutrients, and growth factors, and possibly favoring neural cell regrowth. We have previously demonstrated that a new self-assembling nanostructured biomaterial, based on matrigel, was able to support adult neural stem cell (NSC) culture. In this study, we developed a new 3D cell culture system that takes advantage of the nano- and microfiber assembling process, under physiologic conditions, of these biomaterials. The assembled scaffold forms an intricate and biologically active matrix that displays specifically designed functional motifs: RGD (Arg-Gly-Asp), BMHP1 (bone marrow homing peptide 1), and BMHP2, for the culture of adult NSCs. These scaffolds were prepared at different concentrations, and microscopic examination of the cell-embedded scaffolds showed that NSCs are viable and they proliferate and differentiate within the nanostructured environment of the scaffold. Such a model has the potential to be tailored to develop ad hoc designed peptides for specific cell lines.Keywords: biomaterials, tissue engineering, 3D in vitro model
url http://www.dovepress.com/3d-culture-of-adult-mouse-neural-stem-cells-within-functionalized-self-a7313
work_keys_str_mv AT cunhac 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
AT panseris 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
AT villao 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
AT silvad 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
AT gelainf 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
_version_ 1725629430654566400