Expression of <italic>AmphiNaC,</italic> a new member of the amiloride-sensitive sodium channel related to degenerins and epithelial sodium channels in amphioxus

• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2006-04-01
• Series: International Journal of Biological Sciences
• Subjects: amiloride-sensitive sodium Na⁺; degenerins; <italic>Branchiostoma floridae</italic>; developmental expression; sensory neurons
• Online Access: http://www.biolsci.org/v02p0079.htm

<p>Degenerins and amiloride-sensitive Na+ channels form a new family of cationic ion channels (DEG/NaC). DEG/NaC family emerged as common denominator within a metazoan mechanosensory apparatus. In this study, we characterized a new member of such family in amphioxus, <italic>Branchiostoma floridae</italic>. The <italic>AmphiNaC</italic> cDNA sequence encodes a protein showing amino acid residues characteristic of DEG/NaC family, such as two hydrophobic domains surrounding a large extracellular loop that includes cystein-rich domains; nevertheless its predicted sequence is quite divergent from other family members. <italic>AmphiNaC</italic> is expressed at early larval stage in some putative sensory epidermal cells in the middle of the body and in neurons of the posterior cerebral vesicle, as well as in some ventrolateral and mediolateral neurons of the neural tube. In late larvae, <italic>AmphiNaC</italic> expression is maintained in some neurons of the neural tube, and it is expressed in putative sensory epidermal cells of rostrum and mouth. The analysis of <italic>AmphiNaC</italic> gene expression pattern suggests that it might be involved in neurotransmission and sensory modulation.</p>