Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites

Identifying kinases responsible for specific phosphorylation events remains challenging. Here, the authors leverage kinase inhibitor profiles for the identification of kinase-substrate site pairs in cell extracts, developing a method that can identify the enzymes responsible for unassigned phosphory...

Full description

Bibliographic Details
Main Authors:	Nikolaus A. Watson, Tyrell N. Cartwright, Conor Lawless, Marcos Cámara-Donoso, Onur Sen, Kosuke Sako, Toru Hirota, Hiroshi Kimura, Jonathan M. G. Higgins
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-15428-0

id	doaj-b294ddbbf60f47f4a39a93619abb8a39
record_format	Article
spelling	doaj-b294ddbbf60f47f4a39a93619abb8a392021-05-11T08:37:32ZengNature Publishing GroupNature Communications2041-17232020-04-0111111610.1038/s41467-020-15428-0Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sitesNikolaus A. Watson0Tyrell N. Cartwright1Conor Lawless2Marcos Cámara-Donoso3Onur Sen4Kosuke Sako5Toru Hirota6Hiroshi Kimura7Jonathan M. G. Higgins8Biosciences Institute, Faculty of Medical Sciences, Newcastle UniversityBiosciences Institute, Faculty of Medical Sciences, Newcastle UniversityWellcome Centre for Mitochondrial Research, Faculty of Medical Sciences, Newcastle UniversityBiosciences Institute, Faculty of Medical Sciences, Newcastle UniversityBiosciences Institute, Faculty of Medical Sciences, Newcastle UniversityThe Cancer Institute, Japanese Foundation for Cancer ResearchThe Cancer Institute, Japanese Foundation for Cancer ResearchCell Biology Center, Institute of Innovative Research, Tokyo Institute of TechnologyBiosciences Institute, Faculty of Medical Sciences, Newcastle UniversityIdentifying kinases responsible for specific phosphorylation events remains challenging. Here, the authors leverage kinase inhibitor profiles for the identification of kinase-substrate site pairs in cell extracts, developing a method that can identify the enzymes responsible for unassigned phosphorylation events.https://doi.org/10.1038/s41467-020-15428-0
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nikolaus A. Watson Tyrell N. Cartwright Conor Lawless Marcos Cámara-Donoso Onur Sen Kosuke Sako Toru Hirota Hiroshi Kimura Jonathan M. G. Higgins
spellingShingle	Nikolaus A. Watson Tyrell N. Cartwright Conor Lawless Marcos Cámara-Donoso Onur Sen Kosuke Sako Toru Hirota Hiroshi Kimura Jonathan M. G. Higgins Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites Nature Communications
author_facet	Nikolaus A. Watson Tyrell N. Cartwright Conor Lawless Marcos Cámara-Donoso Onur Sen Kosuke Sako Toru Hirota Hiroshi Kimura Jonathan M. G. Higgins
author_sort	Nikolaus A. Watson
title	Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
title_short	Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
title_full	Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
title_fullStr	Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
title_full_unstemmed	Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
title_sort	kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-04-01
description	Identifying kinases responsible for specific phosphorylation events remains challenging. Here, the authors leverage kinase inhibitor profiles for the identification of kinase-substrate site pairs in cell extracts, developing a method that can identify the enzymes responsible for unassigned phosphorylation events.
url	https://doi.org/10.1038/s41467-020-15428-0
work_keys_str_mv	AT nikolausawatson kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT tyrellncartwright kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT conorlawless kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT marcoscamaradonoso kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT onursen kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT kosukesako kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT toruhirota kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT hiroshikimura kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites AT jonathanmghiggins kinaseinhibitionprofilesasatooltoidentifykinasesforspecificphosphorylationsites
_version_	1721450696927084544

Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites

Similar Items