The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TO...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2017-01-01
|
Series: | Disease Markers |
Online Access: | http://dx.doi.org/10.1155/2017/3160426 |
id |
doaj-b259715ce61e463ea1de62fa63701857 |
---|---|
record_format |
Article |
spelling |
doaj-b259715ce61e463ea1de62fa637018572020-11-25T00:34:20ZengHindawi LimitedDisease Markers0278-02401875-86302017-01-01201710.1155/2017/31604263160426The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryJing Xu0Bin Xu1Chuanhao Tang2Xiaoyan Li3Haifeng Qin4Weixia Wang5Hong Wang6Zhongyuan Wang7Liangliang Li8Zhihua Li9Hongjun Gao10Kun He11Xiaoqing Liu12Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaNational Center of Biomedical Analysis, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Tuberculosis, 309 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Oncology, General Hospital of the PLA Rocket Force, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaNational Center of Biomedical Analysis, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaBackground. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.http://dx.doi.org/10.1155/2017/3160426 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Xu Bin Xu Chuanhao Tang Xiaoyan Li Haifeng Qin Weixia Wang Hong Wang Zhongyuan Wang Liangliang Li Zhihua Li Hongjun Gao Kun He Xiaoqing Liu |
spellingShingle |
Jing Xu Bin Xu Chuanhao Tang Xiaoyan Li Haifeng Qin Weixia Wang Hong Wang Zhongyuan Wang Liangliang Li Zhihua Li Hongjun Gao Kun He Xiaoqing Liu The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Disease Markers |
author_facet |
Jing Xu Bin Xu Chuanhao Tang Xiaoyan Li Haifeng Qin Weixia Wang Hong Wang Zhongyuan Wang Liangliang Li Zhihua Li Hongjun Gao Kun He Xiaoqing Liu |
author_sort |
Jing Xu |
title |
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
title_short |
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
title_full |
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
title_fullStr |
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
title_full_unstemmed |
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
title_sort |
exploration of peptide biomarkers in malignant pleural effusion of lung cancer using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2017-01-01 |
description |
Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE. |
url |
http://dx.doi.org/10.1155/2017/3160426 |
work_keys_str_mv |
AT jingxu theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT binxu theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT chuanhaotang theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT xiaoyanli theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT haifengqin theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT weixiawang theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT hongwang theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT zhongyuanwang theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT liangliangli theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT zhihuali theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT hongjungao theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT kunhe theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT xiaoqingliu theexplorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT jingxu explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT binxu explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT chuanhaotang explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT xiaoyanli explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT haifengqin explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT weixiawang explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT hongwang explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT zhongyuanwang explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT liangliangli explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT zhihuali explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT hongjungao explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT kunhe explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT xiaoqingliu explorationofpeptidebiomarkersinmalignantpleuraleffusionoflungcancerusingmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry |
_version_ |
1725314025485500416 |