The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TO...

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Main Authors: Jing Xu, Bin Xu, Chuanhao Tang, Xiaoyan Li, Haifeng Qin, Weixia Wang, Hong Wang, Zhongyuan Wang, Liangliang Li, Zhihua Li, Hongjun Gao, Kun He, Xiaoqing Liu
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:Disease Markers
Online Access:http://dx.doi.org/10.1155/2017/3160426
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spelling doaj-b259715ce61e463ea1de62fa637018572020-11-25T00:34:20ZengHindawi LimitedDisease Markers0278-02401875-86302017-01-01201710.1155/2017/31604263160426The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryJing Xu0Bin Xu1Chuanhao Tang2Xiaoyan Li3Haifeng Qin4Weixia Wang5Hong Wang6Zhongyuan Wang7Liangliang Li8Zhihua Li9Hongjun Gao10Kun He11Xiaoqing Liu12Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaNational Center of Biomedical Analysis, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Tuberculosis, 309 Hospital, PLA, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaDepartment of Oncology, General Hospital of the PLA Rocket Force, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaNational Center of Biomedical Analysis, Beijing, ChinaDepartment of Pulmonary Oncology, 307 Hospital, PLA, Beijing, ChinaBackground. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.http://dx.doi.org/10.1155/2017/3160426
collection DOAJ
language English
format Article
sources DOAJ
author Jing Xu
Bin Xu
Chuanhao Tang
Xiaoyan Li
Haifeng Qin
Weixia Wang
Hong Wang
Zhongyuan Wang
Liangliang Li
Zhihua Li
Hongjun Gao
Kun He
Xiaoqing Liu
spellingShingle Jing Xu
Bin Xu
Chuanhao Tang
Xiaoyan Li
Haifeng Qin
Weixia Wang
Hong Wang
Zhongyuan Wang
Liangliang Li
Zhihua Li
Hongjun Gao
Kun He
Xiaoqing Liu
The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Disease Markers
author_facet Jing Xu
Bin Xu
Chuanhao Tang
Xiaoyan Li
Haifeng Qin
Weixia Wang
Hong Wang
Zhongyuan Wang
Liangliang Li
Zhihua Li
Hongjun Gao
Kun He
Xiaoqing Liu
author_sort Jing Xu
title The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_short The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_full The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_fullStr The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_full_unstemmed The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_sort exploration of peptide biomarkers in malignant pleural effusion of lung cancer using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
publisher Hindawi Limited
series Disease Markers
issn 0278-0240
1875-8630
publishDate 2017-01-01
description Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method. Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035). Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.
url http://dx.doi.org/10.1155/2017/3160426
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