(Photo)convert to pooled visual screening

(Photo)convert to pooled visual screening

Pooled genetic screening is a powerful method to systematically link genotype to phenotype and gain insights into biological processes, but applying it to visual phenotypes such as cell morphology or protein localization has remained a challenge. In their recent work, Fowler and colleagues (Hasle et...

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Main Authors: Elena Ivanova, Anton Khmelinskii
Format: Article
Language:English
Published: Wiley 2020-06-01
Series:Molecular Systems Biology
Online Access:https://doi.org/10.15252/msb.20209640
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spelling doaj-b24ebb9a93fb47a887634f501e90021a2021-08-02T12:46:24ZengWileyMolecular Systems Biology1744-42922020-06-01166n/an/a10.15252/msb.20209640(Photo)convert to pooled visual screeningElena Ivanova0Anton Khmelinskii1Institute of Molecular Biology (IMB) Mainz GermanyInstitute of Molecular Biology (IMB) Mainz GermanyPooled genetic screening is a powerful method to systematically link genotype to phenotype and gain insights into biological processes, but applying it to visual phenotypes such as cell morphology or protein localization has remained a challenge. In their recent work, Fowler and colleagues (Hasle et al, 2020) describe an elegant approach for high‐throughput cell sorting according to visual phenotypes based on selective photoconversion. This allows combining the advantages of high‐content phenotyping by fluorescence microscopy with the efficiency of pooled screening to dissect complex cellular processes.https://doi.org/10.15252/msb.20209640
collection DOAJ
language English
format Article
sources DOAJ
author Elena Ivanova
Anton Khmelinskii
spellingShingle Elena Ivanova
Anton Khmelinskii
(Photo)convert to pooled visual screening
Molecular Systems Biology
author_facet Elena Ivanova
Anton Khmelinskii
author_sort Elena Ivanova
title (Photo)convert to pooled visual screening
title_short (Photo)convert to pooled visual screening
title_full (Photo)convert to pooled visual screening
title_fullStr (Photo)convert to pooled visual screening
title_full_unstemmed (Photo)convert to pooled visual screening
title_sort (photo)convert to pooled visual screening
publisher Wiley
series Molecular Systems Biology
issn 1744-4292
publishDate 2020-06-01
description Pooled genetic screening is a powerful method to systematically link genotype to phenotype and gain insights into biological processes, but applying it to visual phenotypes such as cell morphology or protein localization has remained a challenge. In their recent work, Fowler and colleagues (Hasle et al, 2020) describe an elegant approach for high‐throughput cell sorting according to visual phenotypes based on selective photoconversion. This allows combining the advantages of high‐content phenotyping by fluorescence microscopy with the efficiency of pooled screening to dissect complex cellular processes.
url https://doi.org/10.15252/msb.20209640
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